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EMAGE:630

Bmp2 bone morphogenetic protein 2 ( MGI:88177)
TS11 (EHF Downs & Davies)
in situ hybridisation

Data Images
EMAGE:630

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Early headfold stage. Anterior is to the left. Extraembryonic region contains reagent trapping.
Expression Pattern Description
Spatial Annotation:
EMAGE:630Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
630_wholemount_strong_3D_1.wlz
630_wholemount_moderate_3D_1.wlz
630_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:630_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
definitive endoderm
strong strong
regionalExpressed in anterior definitive endoderm
embryo ectoderm
not detected not detected
body cavity or lining
not detected not detected
mesenchyme
not detected not detected
organ system
not detected not detected
primitive endoderm
not detected not detected
embryo mesoderm
not detected not detected
notochordal plate
not detected not detected
notochordal process
not detected not detected
primitive streak
not detected not detected
Annotation Validation: submitter
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:11030
Entity Detected:Bmp2, bone morphogenetic protein 2 ( MGI:88177)
Sequence:sense strand is shown

>MGI:11030
AACACCCGGAGAAGGAGGAGGAGGCGAAGAAAAGCAACAGAAGCCCAGTTGCTGCTCCAGGTCCCTCGGA
CAGAGCTTTTTCCATGTGGAGACTCTCTCAATGGACGTGCCCCCTAGTGCTTCTTAGACGGACTGCGGTC
TCCTAAAGCCGCAGGTCGACCATGGTGGCCGGGACCCGCTGTCTTCTAGTGTTGCTGCTTCCCCAGGTCC
TCCTGGGCGGCGCGGCCGGCCTCATTCCAGAGCTGGGCCGCAAGAAGTTCGCCGCGGCATCCAGCCGACC
CTTGTCCCGGCCTTCGGAAGACGTCCTCAGCGAATTTGAGTTGAGGCTGCTCAGCATGTTTGGCCTGAAG
CAGAGACCCACCCCCAGCAAGGACGTCGTGGTGCCCCCCTATATGCTAGATCTGTACCGCAGGCACTCAG
GCCAGCCAGGAGCGCCCGCCCCAGACCACCGGCTGGAGAGGGCAGCCAGCCGCGCCAACACCGTGCGCAG
CTTCCATCAACTAGAAGCCGTGGAGGAACTTCCAGAGATGAGTGGGAAAACGGCCCGGCGCTTCTTCTTC
AATTTAAGTTCTGTCCCCAGTGACGAGTTTCTCACATCTGCAGAACTCCAGATCTTCCGGGAACAGATAC
AGGAAGCTTTGGGAAACAGTAGTTTCCAGCACCGAATTAATATTTATGAAATTATAAAGCCTGCAGCAGC
CAACTTGAAATTTCCTGTGACCAGACTATTGGACACCAGGTTAGTGAATCAGAACACAAGTCAGTGGGAG
AGCTTCGACGTCACCCCAGCTGTGATGCGGTGGACCACACAGGGACACACCAACCATGGGTTTGTGGTGG
AAGTGGCCCATTTAGAGGAGAACCCAGGTGTCTCCAAGAGACATGTGAGGATTAGCAGGTCTTTGCACCA
AGATGAACACAGCTGGTCACAGATAAGGCCATTGCTAGTGACTTTTGGACATGATGGAAAAGGACATCCG
CTCCACAAACGAGAAAAGCGTCAA
nt 197 - nt 1200 of XM_123927.1
Notes:Probe used here corresponds to bases 197-1200 of the BMP2 cDNA (coding sequence) and was originally described by Lyons et al., 1989 [PMID:2481605] . Linearised with XmaIII, transcribed with Sp6.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:EHF Downs & Davies
Theiler Stage:TS11
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Principal investigator:Ruth Arkell, MRC Mammalian Genetics Unit, null, Harwell, UK OX11 ORD
Submitted by:Ruth Arkell, MRC Mammalian Genetics Unit, null, Harwell, UK OX11 ORD
Experiment type:non-screen
References:[ doi:10.1101/gad.3.11.1657] [ PMID:2481605] Lyons KM, Pelton RW, Hogan BL 1989 Patterns of expression of murine Vgr-1 and BMP-2a RNA suggest that transforming growth factor-beta-like genes coordinately regulate aspects of embryonic developm Genes Dev (3):1657-68
Links:MGI:11030 same probe
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE