EMAGE mouse gene expression spatial database

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EMAGE:6349

Magi1 membrane associated guanylate kinase, WW and PDZ domain containing 1 ( MGI:1203522)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_016214. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_016214_01	euxassay_016214_02	euxassay_016214_03	euxassay_016214_04

euxassay_016214_05	euxassay_016214_06	euxassay_016214_07	euxassay_016214_08	euxassay_016214_09

euxassay_016214_10	euxassay_016214_11	euxassay_016214_12	euxassay_016214_13	euxassay_016214_14

euxassay_016214_15	euxassay_016214_16	euxassay_016214_17	euxassay_016214_18	euxassay_016214_19

euxassay_016214_20	euxassay_016214_21	euxassay_016214_22	euxassay_016214_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6349_wholemount_strong.wlz

6349_wholemount_moderate.wlz

6349_wholemount_weak.wlz

6349_wholemount_possible.wlz

6349_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6349_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 02 03 04 05 weak expression: see section 01 06 07 08 19 20 21 22

humerus

moderate

regional

moderate expression: see section 02 03 04

fibula

weak

regional

weak expression: see section 01 02 03 04

tibia

weak

regional

weak expression: see section 02 04

femur

weak

regional

weak expression: see section 03 04 06 07

axial skeleton

moderate

regional

moderate expression: see section 08 09 10 11

clavicle

weak

regional

weak expression: see section 08 09 10

scapula

moderate

regional

moderate expression: see section 04 weak expression: see section 03

pelvic girdle skeleton

weak

regional

weak expression: see section 08 09 10

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T45083
Entity Detected:	Magi1, membrane associated guanylate kinase, WW and PDZ domain containing 1 ( MGI:1203522)
Sequence:	sense strand is shown >T45083 GACAACACTTTGGAAAGAAGGGAGAAGCATGAGAAGAGAAGAGAGATATCTCCCGAGAGGAAGCGAGAGC GTTCACCCACCCGGAGAAAAGATAGCTCCCCGAGCCGCCGGCGGAGGTCCCTCGAAAGACTCCTGGATCA AAGACGGTCCCCAGAGCGCAGAAGAGGGGGCTCCCCCGAGAGGAGAGCCAAATCCACCGACAGGAGGAGG GCCAGGTCCCCTGAGCGCAGGCGAGAGCGGTCACTGGACAAAAGGAACCGGGACGACAAGGTTGGCCACC GAGAAAGAGAGGAGGCTGGTCTGAAGCTGGAAGCGGGGAGAAGCCCCCGAAATCCCCCAGAGCAGAGAAG GCGGCCTTACAAAGAATGTAGCACCGACCTCAGCATCTGAGACTCAGCATTGCAACCTTTACCGTGTC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from genomic DNA prepared from tail-tips of two wild-type C57BL/6J mice), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 212687. Forward Primer - name:212687_F_exon_Baiap1, sequence:GACAACACTTTGGAAAGAAGGG; Reverse Primer - name:212687_N_SP6_exon_Baiap1, sequence:GACACGGTAAAGGTTGCAATG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6350 same embryo
	EMAGE:6352 same embryo
	EMAGE:6351 same embryo
	EurExpress:euxassay_016214 same experiment
	MGI:4826063 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source