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EMAGE:6361

Celf2 CUGBP, Elav-like family member 2 ( MGI:1338822)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:6361 EMAGE:6361 EMAGE:6361 EMAGE:6361 EMAGE:6361
"Pseudo-wholemount" of euxassay_016197. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_016197_01 euxassay_016197_02 euxassay_016197_03 euxassay_016197_04
EMAGE:6361 EMAGE:6361 EMAGE:6361 EMAGE:6361 EMAGE:6361
euxassay_016197_05 euxassay_016197_06 euxassay_016197_07 euxassay_016197_08 euxassay_016197_09
EMAGE:6361 EMAGE:6361 EMAGE:6361 EMAGE:6361 EMAGE:6361
euxassay_016197_10 euxassay_016197_11 euxassay_016197_12 euxassay_016197_13 euxassay_016197_14
EMAGE:6361 EMAGE:6361 EMAGE:6361 EMAGE:6361 EMAGE:6361
euxassay_016197_15 euxassay_016197_16 euxassay_016197_17 euxassay_016197_18 euxassay_016197_19
EMAGE:6361 EMAGE:6361 EMAGE:6361 EMAGE:6361
euxassay_016197_20 euxassay_016197_21 euxassay_016197_22 euxassay_016197_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:6361Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
6361_wholemount_strong.wlz
6361_wholemount_moderate.wlz
6361_wholemount_weak.wlz
6361_wholemount_possible.wlz
6361_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:6361_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T45401
Entity Detected:Celf2, CUGBP, Elav-like family member 2 ( MGI:1338822)
Sequence:sense strand is shown

>T45401
GAATTCTGCTCAAGTCCTTGCTGTCTGTTTACAAAGGGGAGCTCAGAATGCCTATAGGTCCATTGTCGTC
CCCCCCTCCCCCTCCCCCCCCCCCACCGCATTTGACACAGGATAGGAAGGAGATGTGCTCTCACTGTACC
CAACGCCTGATCAGAGAAGAACTGCCCCACTGCCCATGCTGTGACAAGCCCTCCCCGCTCTACTGTCTAC
TTGCTCAGGAGTCTGGCTGCCCTCAAAGAGTATTTCCCATGGGTATCTGCTCCCTTCTCTTCTCGCCCGT
GCCAGGACACCTGAGCTGGTGTCAGCGAAAGCTCTGGCTGCCCACCCTCTGGTTTCAGCTGGCCCCTCAG
GCCCCTTTCTTCAGTCAGGTCAACAACCATGGGCCTACCAAACAGAAGAAAGGTGGCTCTTTCCCTAGGC
CAAACCTCTCACTGGGCATGAGGTTCCGAGCCCTGCTCCTACTCTGACAAAAGCTGATTCCTAGTGACAC
CTGTGCTGGCTTTCTC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from genomic DNA prepared from tail-tips of two wild-type C57BL/6J mice), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 193619. Forward Primer - name:193619_F_exon_B230218O03, sequence:GAATTCTGCTCAAGTCCTTGCT; Reverse Primer - name:193619_N_SP6_exon_B230218O03, sequence:GAGAAAGCCAGCACAGGTGTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:6363 same embryo
 EMAGE:6362 same embryo
 EMAGE:6364 same embryo
 EurExpress:euxassay_016197 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS