EMAGE mouse gene expression spatial database

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EMAGE:6374

Epha5 Eph receptor A5 ( MGI:99654)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_016273. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_016273_01	euxassay_016273_02	euxassay_016273_03	euxassay_016273_04

euxassay_016273_05	euxassay_016273_06	euxassay_016273_07	euxassay_016273_08	euxassay_016273_09

euxassay_016273_10	euxassay_016273_11	euxassay_016273_12	euxassay_016273_13	euxassay_016273_14

euxassay_016273_15	euxassay_016273_16	euxassay_016273_17	euxassay_016273_18	euxassay_016273_19

euxassay_016273_20	euxassay_016273_21	euxassay_016273_22	euxassay_016273_23	euxassay_016273_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6374_wholemount_strong.wlz

6374_wholemount_moderate.wlz

6374_wholemount_weak.wlz

6374_wholemount_possible.wlz

6374_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6374_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

facial vii ganglion

moderate

single cell

moderate expression: see section 04

glossopharyngeal ix ganglion

moderate

single cell

moderate expression: see section 05 06 16 17 18

trigeminal v ganglion

moderate

single cell

moderate expression: see section 04 05 06 08 16 17 18 20 21 weak expression: see section 19

vestibulocochlear viii ganglion

moderate

single cell

moderate expression: see section 05 06 17

dorsal root ganglion

moderate

single cell

moderate expression: see section 05 06 07 08 09 12 13 14 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T45064
Entity Detected:	Epha5, Eph receptor A5 ( MGI:99654)
Sequence:	sense strand is shown >T45064 CTCCAAGTGAACAACTCAGCACTTTGTAAACAACCCTGAGATTTATTTTAACAGAGAAAGGGGAAAGGGT GGTTTCTAAACCTTTGGAGGCATTTGCCTTAACCTTTGAGTTTATAATCAATATTTTACTAAAATCTCCT GATCTTCCTCTTAATTCCACAATGTACAGGTAACCTGCAAAGAGAGCTAACATGACGATCAAACATCCTT TATTAAAATATGTAACGAAATCTTCCCCACTTCTTCCATGGAGTCTTGTACAGGAAATGTATCAAGCTAG AGCACCTTTAGAGACTGTTAAGGCAGCCCCTTTCAAAACTTCCAGGGATCAACTTGAAAGGAAAAGTTTT AAAGCCATGTGTGGGCTAAGAAAAGCTGCATTTTACTGACGTTTACTTCAAGTCTTAATTGTCTACATGA GTGTATTGAAGAGCAATATGATTAGATTATTTCTTAAATAGACTTTGTAATTTAAAATGAAATTGCATGT TGTTAAGTTATAGAAGATAGTTTATAAACATGTTGCTTGGTCAAGGAAAAGTTCAATACAGGGTGTATAT TTATTTTTCTGTGTTAGAAAATTTACTTTTAGTTGCTCTTCTAGAGAGTATTAGGTAATGAATGTGTATA TATGTATAGTTTGCAATATATGCAGGAACTGACTTACATCGAAATTGTGTGTGTGTGTGTGTGCGTCGGT GCGAGAGTATGTGTAGTATTTGAATATGTGGAGGGTATTTTTTGCTTGCCTTTTGGATAGTGTTTTAATT TTGACGACATACAGGAAAGTGTCTTCTAGACTCTATCATGGGTAAAAGAGTAGAAAGCCGTAGACCAAAT TCCCATCCAAGTTGGTATCTTATGCTCTCATTTTTAAAGCAACTAATTAGTTCATTTAAACAGCTAATGC CTCTAGACTAAATTCCCTTTGG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from genomic DNA prepared from tail-tips of two wild-type C57BL/6J mice), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 226339. Forward Primer - name:226339_F_exon_Epha5, sequence:CTCCAAGTGAACAACTCAGCAC; Reverse Primer - name:226339_N_SP6_exon_Epha5, sequence:CCAAAGGGAATTTAGTCTAGAGG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6370 same embryo
	EMAGE:6371 same embryo
	EMAGE:6375 same embryo
	EMAGE:6372 same embryo
	EMAGE:6373 same embryo
	EurExpress:euxassay_016273 same experiment
	MGI:4824582 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source