EMAGE mouse gene expression spatial database

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EMAGE:6386

2310065F04Rik RIKEN cDNA 2310065F04 gene ( MGI:1921434)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_016255. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_016255_01	euxassay_016255_02	euxassay_016255_03	euxassay_016255_04

euxassay_016255_05	euxassay_016255_06	euxassay_016255_07	euxassay_016255_08	euxassay_016255_09

euxassay_016255_10	euxassay_016255_11	euxassay_016255_12	euxassay_016255_13	euxassay_016255_14

euxassay_016255_15	euxassay_016255_16	euxassay_016255_17	euxassay_016255_18	euxassay_016255_19

euxassay_016255_20	euxassay_016255_21	euxassay_016255_22	euxassay_016255_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6386_wholemount_strong.wlz

6386_wholemount_moderate.wlz

6386_wholemount_weak.wlz

6386_wholemount_possible.wlz

6386_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6386_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

weak

regional

weak expression: see section 09 10 15 16

pons mantle layer

weak

regional

weak expression: see section 08 16

facial vii ganglion

moderate

regional

moderate expression: see section 05 19

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 17 weak expression: see section 18

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 15 16 17 19 weak expression: see section 18 20

vagus x ganglion

moderate

regional

moderate expression: see section 08 16

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 07 17

ventral grey horn

moderate

single cell

moderate expression: see section 10 11 12 13 14 15

dorsal root ganglion

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18

neural retina

moderate

regional

moderate expression: see section 01 weak expression: see section 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T45603
Entity Detected:	2310065F04Rik, RIKEN cDNA 2310065F04 gene ( MGI:1921434)
Sequence:	sense strand is shown >T45603 CAGGAAGCTTTGAGGTTTGAGTCCGCTTGGCAGGGAGGAGGCGTCACTCAGGGAGCATGGACTGTGTTCT GGGGATTTGAGATTGGGGGACTGTTCTCAAGAGAGACCTGGGGTTGTGCTCAGCCTTGCCCAATGCTTGG GTCACTCTAGTCTGGAATGGTCACAGAAGTGCAGTCTCTGTCGGAGAAACCAAACCCTTTGTAGGACCTG TCTTGAACTAAGGAAAGAATGGTAGGTGGAACAGAGCTCAACGGACACCGAGAGCATCTCCCAAGCGACA AGACCCCCCCCCCCGGCCCCCGTTAGTGAGCGTGAGATGTTCGTGGGCAAAGACACGGGAATGGGTTCTC AAGCAGAGGGGATGCACTTTATGATCCATCTGCCATGTTTGCAGGTGAGTGAGTGAGG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from genomic DNA prepared from tail-tips of two wild-type C57BL/6J mice), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 209187. Forward Primer - name:209187_F_exon_2310065F04Rik, sequence:CAGGAAGCTTTGAGGTTTGAGT; Reverse Primer - name:209187_N_SP6_exon_2310065F04Rik, sequence:CCTCACTCACTCACCTGCAAA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6388 same embryo
	EMAGE:6387 same embryo
	EMAGE:6384 same embryo
	EMAGE:6385 same embryo
	EMAGE:6389 same embryo
	EurExpress:euxassay_016255 same experiment
	MGI:4822669 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source