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EMAGE:6389

Dyrk2 dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 2 ( MGI:1330301)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:6389 EMAGE:6389 EMAGE:6389 EMAGE:6389 EMAGE:6389
"Pseudo-wholemount" of euxassay_016272. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_016272_01 euxassay_016272_02 euxassay_016272_03 euxassay_016272_04
EMAGE:6389 EMAGE:6389 EMAGE:6389 EMAGE:6389 EMAGE:6389
euxassay_016272_05 euxassay_016272_06 euxassay_016272_07 euxassay_016272_08 euxassay_016272_09
EMAGE:6389 EMAGE:6389 EMAGE:6389 EMAGE:6389 EMAGE:6389
euxassay_016272_10 euxassay_016272_11 euxassay_016272_12 euxassay_016272_13 euxassay_016272_14
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euxassay_016272_15 euxassay_016272_16 euxassay_016272_17 euxassay_016272_18 euxassay_016272_19
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euxassay_016272_20 euxassay_016272_21 euxassay_016272_22 euxassay_016272_23 euxassay_016272_24
EMAGE:6389
euxassay_016272_25

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:6389Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
6389_wholemount_strong.wlz
6389_wholemount_moderate.wlz
6389_wholemount_weak.wlz
6389_wholemount_possible.wlz
6389_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:6389_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
cerebral cortex mantle layer
moderate moderate
regionalmoderate expression: see section 05 06 07 08 09 10 11 12 21 22 weak expression: see section 02 03 04 13 14 15 16 17 18 19 20 23 24
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T45541
Entity Detected:Dyrk2, dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 2 ( MGI:1330301)
Sequence:sense strand is shown

>T45541
CTATGAACGTCATCCACATGCTGGAGAACTTCACCTTCCGCAACCACATCTGCATGACGTTCGAGTTGCT
GAGCATGAACCTCTATGAGCTCATCAAGAAGAATAAGTTCCAGGGCTTCAGCCTGCCTCTGGTGCGCAAG
TTTGCCCACTCCATTCTGCAGTGCTTGGATGCTTTGCACAAGAACAGAATAATCCACTGTGACCTTAAGC
CCGAGAACATTTTGTTAAAGCAGCAGGGTCGAAGCAGTATTAAAGTGATTGACTTTGGCTCCAGTTGTTA
CGAGCACCAACGCGTCTACACGTACATCCAGTCACGCTTTTACCGGGCTCCGGAAGTGATCCTCGGAGCC
AGGTATGGCATGCCCATAGACATGTGGAGCCTGGGTTGCATCCTCGCGGAACTCCTGACGGGTTACCCCC
TCTTGCCTGGGGAGGATGAAGGGGACCAGCTGGCTTGTATGATCGAGCTTTTGGGCAT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from genomic DNA prepared from tail-tips of two wild-type C57BL/6J mice), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 204917. Forward Primer - name:204917_F_exon_1810038L18Rik, sequence:CTATGAACGTCATCCACATGCT; Reverse Primer - name:204917_N_SP6_exon_1810038L18Rik, sequence:ATGCCCAAAAGCTCGATCATA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:6388 same embryo
 EMAGE:6387 same embryo
 EMAGE:6386 same embryo
 EMAGE:6384 same embryo
 EMAGE:6385 same embryo
 EurExpress:euxassay_016272 same experiment
 MGI:4824436 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS