EMAGE mouse gene expression spatial database

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EMAGE:6419

Dnmbp dynamin binding protein ( MGI:1917352)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_016234. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_016234_01	euxassay_016234_02	euxassay_016234_03	euxassay_016234_04

euxassay_016234_05	euxassay_016234_06	euxassay_016234_07	euxassay_016234_08	euxassay_016234_09

euxassay_016234_10	euxassay_016234_11	euxassay_016234_12	euxassay_016234_13	euxassay_016234_14

euxassay_016234_15	euxassay_016234_16	euxassay_016234_17	euxassay_016234_18	euxassay_016234_19

euxassay_016234_20	euxassay_016234_21	euxassay_016234_22	euxassay_016234_23	euxassay_016234_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6419_wholemount_strong.wlz

6419_wholemount_moderate.wlz

6419_wholemount_weak.wlz

6419_wholemount_possible.wlz

6419_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6419_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

lens

moderate

regional

moderate expression: see section 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T45578
Entity Detected:	Dnmbp, dynamin binding protein ( MGI:1917352)
Sequence:	sense strand is shown >T45578 TATGGAATCGCCCTCTACAGATTCCAAGCCCTGGAGACGAATGAGTTGGACTTTGAGGTAGGGGACAGAA TTCAAATACTGGGGACCTTGGAGGATGGCTGGCTGGAAGGATGTCTGAAGGGCAAGACAGGGGTCTTTCC CCATCGCTTTGTGAAGTTATGTCCTAGCAACAGGACAGAGGAAACCACGGCTCAGCCCCAGGAAAGCAGT TTCCCCAAGGACTCTGAAAGCTCTGTGGGCAAGTCAGGGGACTCCGTGGTGGAGGAAGCAAGACAGGAAC CATGGGAGTGTGAAGAAGAAAGACCTGACTATGATCTCCCAGGACAAGCCTCCGTGCCCCAAGATCACGT AGCACCCGAGTGGACTGGAGATACGATCTCAGGTCAGGATAAAGATGCTTCAGGGAGCTCTCCTGATGTG GATCTTGAAAGGCCTCTTGCTAAAGACCTTTCCACACCTGACCCCTCAGAGGAAGTCAACGGTGTTTCTT CCCAGCCTCAGGTACCTATTCATCCCAAAGTGCAGAAGAGCCAACACTATCTAACAGCAGGAGGGAGCCA CCAAACCTCTGACCCATTCTCTGAGCTTGTCCCACTAGAAGCCAGGACCAGAGACTACTCCAGCCTGCCT C
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from genomic DNA prepared from tail-tips of two wild-type C57BL/6J mice), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 181604. Forward Primer - name:181604_F_exon_Dnmbp, sequence:TATGGAATCGCCCTCTACAGAT; Reverse Primer - name:181604_N_SP6_exon_Dnmbp, sequence:GAGGCAGGCTGGAGTAGTCTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6420 same embryo
	EMAGE:6417 same embryo
	EMAGE:6415 same embryo
	EMAGE:6418 same embryo
	EMAGE:6416 same embryo
	EurExpress:euxassay_016234 same experiment
	MGI:4824364 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source