EMAGE mouse gene expression spatial database

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EMAGE:6431

Chmp2b charged multivesicular body protein 2B ( MGI:1916192)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_016382. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_016382_01	euxassay_016382_02	euxassay_016382_03	euxassay_016382_04

euxassay_016382_05	euxassay_016382_06	euxassay_016382_07	euxassay_016382_08	euxassay_016382_09

euxassay_016382_10	euxassay_016382_11	euxassay_016382_12	euxassay_016382_13	euxassay_016382_14

euxassay_016382_15	euxassay_016382_16	euxassay_016382_17	euxassay_016382_18	euxassay_016382_19

euxassay_016382_20	euxassay_016382_21	euxassay_016382_22	euxassay_016382_23	euxassay_016382_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6431_wholemount_strong.wlz

6431_wholemount_moderate.wlz

6431_wholemount_weak.wlz

6431_wholemount_possible.wlz

6431_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6431_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

weak

regional

weak expression: see section 04 05 21

medulla oblongata basal plate mantle layer

weak

regional

weak expression: see section 06 07 08 15 17

rest of cerebellum mantle layer

strong

regional

strong expression: see section 10 11 14 moderate expression: see section 09 13 15 weak expression: see section 05 06 08 17

rest of cerebellum ventricular layer

strong

regional

strong expression: see section 10

intermediate grey horn

moderate

regional

moderate expression: see section 08 09 10 11 12 13 weak expression: see section 07 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T63071
Entity Detected:	Chmp2b, charged multivesicular body protein 2B ( MGI:1916192)
Sequence:	sense strand is shown >T63071 GATTGGTAATAAGGAAGCGTGCAGGGTTTTAGCTAAGCAGCTTGTCCACCTACGGAAACAGAAGACAAGA ACGTTCGCTGTAAGCTCAAAAGTCACCTCTATGTCCACACAAACGAAAGTGATGAATTCCCAGATGAAGA TGGCCGGTGCCATGTCTACCACTGCAAAGACAATGCAAGCAGTCAACAAGAAAATGGATCCACAGAAGAC ACTACAAACAATGCAGAATTTCCAGAAGGAAAACATGAAAATGGAAATGACTGAAGAAATGATCAATGAC ACCCTGGATGACATCTTTGATGGCTCTGATGATGAAGAAGAAAGCCAGGACATCGTGAATCAAGTTCTTG ATGAAATTGGGATTGAAATCTCTGGAAAGATGGCCAAAGCTCCATCGGCTGCAAGAAGCTTACCGTCTGC CTCCACTTCAAAGGCTACAATTTCTGATGAAGAGATTGAACGTCAACTCAAAGCTTTAGGGGTAGATTAG ATCAAAAGCCATACATACTGTTCTCACTTGAGTCTAAAGTGAGTCCGGTGTAGATTCCTTTTACAAAACA CATTCATTTTGCAAAAATGAAGACCATGAGTGAACAGTTATTTCCTGACCCATGGCTACTTTGAATCCTT TGCCAAAGAGTGCCAATGATGCAA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 141990. Forward Primer - name:141990_F_cDNA_1190006E07Rik, sequence:GATTGGTAATAAGGAAGCGTGC; Reverse Primer - name:141990_N_SP6_cDNA_1190006E07Rik, sequence:TTGCATCATTGGCACTCTTTG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6430 same embryo
	EMAGE:6429 same embryo
	EurExpress:euxassay_016382 same experiment
	MGI:4823849 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source