EMAGE mouse gene expression spatial database

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EMAGE:6500

Mir674 microRNA 674 ( MGI:3629631)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019057. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019057_01	euxassay_019057_02	euxassay_019057_03	euxassay_019057_04

euxassay_019057_05	euxassay_019057_06	euxassay_019057_07	euxassay_019057_08	euxassay_019057_09

euxassay_019057_10	euxassay_019057_11	euxassay_019057_12	euxassay_019057_13	euxassay_019057_14

euxassay_019057_15	euxassay_019057_16	euxassay_019057_17	euxassay_019057_18	euxassay_019057_19

euxassay_019057_20	euxassay_019057_21	euxassay_019057_22	euxassay_019057_23	euxassay_019057_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6500_wholemount_strong.wlz

6500_wholemount_moderate.wlz

6500_wholemount_weak.wlz

6500_wholemount_possible.wlz

6500_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6500_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

cerebral cortex mantle layer

moderate

regional

moderate expression: see section 13 14 15 16 21 22 23 weak expression: see section 02 04 05 06 17 18 19 20

telencephalon mantle layer

moderate

regional

moderate expression: see section 21 22 23 weak expression: see section 05 06 19 20

olfactory cortex mantle layer

moderate

regional

moderate expression: see section 10 15 16 17 weak expression: see section 09 11

trigeminal v ganglion

weak

single cell

weak expression: see section 03 04 05 06 07 08 09 17 18 19 20 21

dorsal grey horn

weak

regional

weak expression: see section 10 11 12 14 15 16

ventral grey horn

weak

regional

weak expression: see section 10 11 12 14 15 16

dorsal root ganglion

weak

single cell

weak expression: see section 07 08 09 13 14 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T70122
Entity Detected:	Mir674, microRNA 674 ( MGI:3629631)
Sequence:	sense strand is shown >T70122 GCACTGAGATGGGAGTGGTGTA
Notes:	The probe used by the EURExpress consortium to detect the presence of miRNA mmu-miR-674 was custom designed and ordered from Sigma-Exiqon by the EURExpress partner in Geneva and then distributed to the ISH generating units within the consortium.
Chemistry:	LNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6499 same embryo
	EurExpress:euxassay_019057 same experiment
	MGI:4826357 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source