EMAGE mouse gene expression spatial database

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EMAGE:6533

Fbxo45 F-box protein 45 ( MGI:2447775)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_016469. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_016469_01	euxassay_016469_02	euxassay_016469_03	euxassay_016469_04

euxassay_016469_05	euxassay_016469_06	euxassay_016469_07	euxassay_016469_08	euxassay_016469_09

euxassay_016469_10	euxassay_016469_11	euxassay_016469_12	euxassay_016469_13	euxassay_016469_14

euxassay_016469_15	euxassay_016469_16	euxassay_016469_17	euxassay_016469_18	euxassay_016469_19

euxassay_016469_20	euxassay_016469_21	euxassay_016469_22	euxassay_016469_23	euxassay_016469_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6533_wholemount_strong.wlz

6533_wholemount_moderate.wlz

6533_wholemount_weak.wlz

6533_wholemount_possible.wlz

6533_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6533_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 08 09 15 16

facial vii ganglion

weak

regional

weak expression: see section 05

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 06 17 18

trigeminal v ganglion

moderate

regional

moderate expression: see section 09 weak expression: see section 04 05 06 07 08 17 18 19 21 22

vagus x ganglion

weak

regional

weak expression: see section 07

ventral grey horn

moderate

regional

moderate expression: see section 09 10 weak expression: see section 12 13 14

dorsal root ganglion

moderate

regional

moderate expression: see section 07 weak expression: see section 06 08 09 10 11 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7844
Entity Detected:	Fbxo45, F-box protein 45 ( MGI:2447775)
Sequence:	sense strand is shown >T7844 GCGGGAACCCGAGCGGCTCGCGCCATCTGCCCTCAGTGAGGCGGGGCGCGCAGCGGACGCCCCGGGGCAG GGGCGGGAGCGGTGGAGGCGCGAGCGGCTGGCGCTGAAAGGGGCGGTGAGCGAGCCGCTCGCGGCTGTAG GCGAGGCTTGGCCTCCCGCGCCGAGACGGGGCGAAGCGGCGGCGGCGGCGGCCCTCGGGCCGGCTGGTGA GGCGATGGCGGCGCCGGGCCCGGGGGCTGGGGCAGCCTCGGGCGGCGCTAGTGGTGGCGGCGCCGGTGCC GGCGGCGGCGCCAGCGCGGGCTCGGGGTCCTCCGGGGTCGGCGGCCGGTTGCCCAGCCGGGTGCTGGAGT TGGTGTTCTCCTACCTGGAGCTGTCCGAGCTGCGGAGCTGCGCCCTGGTTTGCAAGCACTGGTACCGCTG CCTCCACGGCGACGAGAACAGCGAGGTGTGGCGGAGCCTGTGCGCCCGCAGCCTAGCAGAAGAGGCTCTG CGCACGGACATCCTCTGCAACCTGCCCAGCTACAAGGCCAAGGTGCGTGCTTTCCAACATGCCTTCAGCA CGAATGACTGCTCCAGGAATGTCTATATCAAGAAGAATGGTTTTACATTACATCGGAACCCCATCGCTCA GAGCACTGATGGTGCAAGGACCAAGATTGGTTTCAGTGAGGGCCGCCACGCCTGGGAAGTGTGGTGGGAG GGCCCTTTGGGCACGGTGGCAGTGATTGGGATTGCTACGAAACGGGCCCCCATGCAGTGCCAAGGTTATG TGGCCTTGCTGGGAAGTGACGACCAGAGCTGGGGCTGGAATCTGGTGGACAATAATCTACTACATAACGG GGAAGTCAACGGCAGCTTCCCACAGTGCAACAACGCACCAAAGTATCAGATAGGAGAGAGAATCCGAGTC ATCCTGGACATGGAGGATAAGACTTTAGCTTTTGAGCGTGGGTATGAGTTCCTGGGGGTTGCCTTTCGAG GACTTCCAAAAGCCTGCTTATACCCAGCTGTCTCTGCAGTATATGGCAACACAGAAGTGACTTTGGTTTA TCTTGGAAAACCTCTGGATGGATGACAGTGACTTTCTGGAAAGAAGAAGAGTGGAGAAGAGATCTACTTG TGGAAAGTAGAACCATGAAGTGATAGTATCATGTGTGCATGCCCAAGAAACATCCTGAAGACACGTGAAG TTGAGAACTGGAGAAGCAGCTCTGCCACAGAGTGATCATAGCGTTTCCTCTTTCCACTGGGCCAGAAAAT CCTCAGGGTTGCAGTTGGTTGAGTGGGCAGTTGACATATGCATGTTGCCACAGCGTCTTCTCTAAGTTAG CAGTGTGTTACTTCCAGCTTAAAGGTGAGCGTGTAGAGATGCCATAGGAGGGAATCGCAAGGGTTTATGT AGTGTTGATGGAGAAGGCTCGGTCCCATCTTACAACTGCCTGTTCTTTCTCCAGTCCCTTTATTTCTAGC CAGCTTGACTTAGAAAGTATGAAACTGGCTGGGTTTTATTTAATATTTTTAATATATTGAGAAGCATGGT CTGCCTGGACTGCACTTCTCTAGCAATGAGAAATAAAATTGTGCAGCTATTTTAAAAGTTGTATATATAA TGTGTGTAAAAACTGTATAAAAAGGGGGGGGGGACAAAGGCATTGCTTTGTTCTACTTCAATTTCTTAAA AATCACTACATGGTACAAAATTACAGTGACATTTGGGGATAGTAGGGTAACAGAGAAAAGAAGAGGAGAC ATGTTGTACTGGTTCATTTGATAGTGTTTTGGGCTTATAGTCCATGGCTATAACAGGATTATGATAAAGG TTCCTCAATGGTTAACGCCTGGGGAAGCAGATCTACTTAGCATTGTGCACATGCCAGCAAGGCTGACTCA GAAGTGATAGATAGGAACGTGCCTCTTGGCATCTGGCTTAGTTTTTGGCCTTCTGAGAAAACTGGTCTCC ACACAGAACAGTCTCAGCTGATCTTACAGAACTCAGTGTGAGCGCTGTTAGTATAAACCCTAGGAAAACA CATGGTCAGCCATTCTCTCATTTCTTAACTTTATAATGCTGTAGCTGTTATGAAGAATTTTTAATTTCAT ATTGTTTACATTATACAACAATCGAAGCCTCAAAGTCTCCACTGGGGCTGTCATCAAATGTTTACTCACC CACCTGAGACAGTGCCAGCCTGAGCCCAGGTTAGGAGTGTTCTTAGTCACCTAGGTGTTGCCCCATGGGA CTTAAGGCTGAAACCTTCAGGTTGGCAGCACCATGAAAGCTTCCTTGGCAAGAGTGAACGTCAGCTCCTA TTCTGACCCGTAACCTTCTCACTAATACTCTCTGGAGTGACTCAACACATGGGCTGAGGTGGTTGTTGGT TGTTTTCCTTTTGTTAGAACAGAAACGTCTTTCTGTATAAAAGTATAATTAATATGGTTTAGATACATAA GAATTGATGAAGGCAAGTAAAATCTTTGTACTTTTTACATGATTGCTGTATGTATATTTTGTTTTAAATC TAATAAAATTAGGGACAGAGAGTGACTCGCTGGGGTTCTTGGGGTCCTTGAGAATTAAATTTGTCAAAAC TGCTATGAAACAGGAGACTGGCCACAGGACGGTCTGTAGTTGCCTCCTGAAAGGGTTACATGTATTTATT GTATATTTGCTATGTTGCTTGAAAAAGAAATTTGTTAAAGATTCTTTTTGATAATCTGTATCAAGAAGAA ACCTTGTACAAAGTGGAATTATATGTTAAACGTTTTAGGGAAGATAGCTGGCCCAGGAATTGTTTTGTTT TTCTTGTTTTTTGTGGGTTTTTTGTTTGTTTTTCTGCTCTAGAACAACTCAATTTGGTGGCACTTAAAAA TTGATTAAAGACCTGGGTAAGGATGAAGACTGACTAGTTAAAAGGTGCGGGTGGTCCTTACTCGCTGCAT CTTCAGGGTATTACAGTTCCTCAAGTTCTCATGAGTTTGACTCAGAACTTCTGTTTTTGTCTTGTTGTTG GTAAATGTTCATTTGCAACAAGTTTTTGAAGGTGGTGACTGGAAAACAAAATCACATGGTTATTCATTCC TCATTGAACTTAGAGTCGGATAATTGTCTAACAATTTAACACCAACCCACTCTTTCTGTGTCTTGTTTTG ATTTTGGTTTTTTTAAAAGCTGAACTCACAGCGATCTCCCTGCGTCAACCTCCCCAAAACTGGGATTAGA GGGAATGCTACCATTCCCAGAACAACAAGCCATTTTAGCTCAACGATTTGAGTGTTCCTAGTCAGGACAG GCCTCTAGAGAACAGAGAACCTTCATTTATAGCCTGATCTATTTACTAGAAATCTTGTTAGTGAGATAAG TAGTGGCTGCCCAGATCATTAAGGTAAACTGCTGAGTGTTAAAGGAGGTCGTGCTCTTGGTGGTGCGTAA AGAGCACATGGAAGACACAGTTGTACCTGAGCTGAGTATGAGTCACCTGAGTCAGTGCTGTTCAAACATG GCCAGATCTCAGAGTGGCTTAAAAGTACTGTGGATGGGTCCGTATCCTGATCCTGGTGTCAGAACTGGGA TATGTTCCGACCATAAGGATTTAAAAATGCCCTGGTTTCAGAATGCTACCAAAGGTTGAGAGCAGTTGTG TCGAATTTGACCTAGAAGTTTCTCACCTACAAAGTAGGAAGTTCTTGCCCCACTCATTGCCTCACGGGGA TGCAGAGGGCGCAAGAGAACACTGGGAGTGGTGGCGGTATGGATGAACAATGGTGCAGAAGTGTAGCATC TGTCACATTCCAGCTGCAGCCACCGTGACCAGGGAAACGTGTCGTTGAATTTGTTGTTGCTGTAGGAATC TCTTACGTCATGCATAAGGAAGCATGCAGGGGTGGAACAAAATGTTAATTAATATTACTCTTATTCACTA TGGCCTTCTGTCCTACTTGAGACAGGCGGTTATATTACCTAAGTTTTCATAGGAAAGAAGAAATATTAAG GCTTAATTCTGTAATGTTCATTGGCTCATAATTCATTAAAAACTTCATACAAGGAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6337188 using vector (pCMV-SPORT6.1) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6337188 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6535 same embryo
	EMAGE:6537 same embryo
	EMAGE:6536 same embryo
	EMAGE:6532 same embryo
	EMAGE:6534 same embryo
	EurExpress:euxassay_016469 same experiment
	MGI:4824810 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source