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EMAGE:6534

Mir222 microRNA 222 ( MGI:3619118)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:6534 EMAGE:6534 EMAGE:6534 EMAGE:6534 EMAGE:6534
"Pseudo-wholemount" of euxassay_019037. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_019037_01 euxassay_019037_02 euxassay_019037_03 euxassay_019037_04
EMAGE:6534 EMAGE:6534 EMAGE:6534 EMAGE:6534 EMAGE:6534
euxassay_019037_05 euxassay_019037_06 euxassay_019037_07 euxassay_019037_08 euxassay_019037_09
EMAGE:6534 EMAGE:6534 EMAGE:6534 EMAGE:6534 EMAGE:6534
euxassay_019037_10 euxassay_019037_11 euxassay_019037_12 euxassay_019037_13 euxassay_019037_14
EMAGE:6534 EMAGE:6534 EMAGE:6534 EMAGE:6534 EMAGE:6534
euxassay_019037_15 euxassay_019037_16 euxassay_019037_17 euxassay_019037_18 euxassay_019037_19
EMAGE:6534 EMAGE:6534 EMAGE:6534 EMAGE:6534 EMAGE:6534
euxassay_019037_20 euxassay_019037_21 euxassay_019037_22 euxassay_019037_23 euxassay_019037_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:6534Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
6534_wholemount_strong.wlz
6534_wholemount_moderate.wlz
6534_wholemount_weak.wlz
6534_wholemount_possible.wlz
6534_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:6534_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T70348
Entity Detected:Mir222, microRNA 222 ( MGI:3619118)
Sequence:sense strand is shown

>T70348
AGCTACATCTGGCTACTGGGT
Notes:The probe used by the EURExpress consortium to detect the presence of miRNA mmu-miR-222 was custom designed and ordered from Sigma-Exiqon by the EURExpress partner in Geneva and then distributed to the ISH generating units within the consortium.
Chemistry:LNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:6535 same embryo
 EMAGE:6533 same embryo
 EMAGE:6537 same embryo
 EMAGE:6536 same embryo
 EMAGE:6532 same embryo
 EurExpress:euxassay_019037 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS