EMAGE mouse gene expression spatial database

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EMAGE:6559

Mtap7d1 microtubule-associated protein 7 domain containing 1 ( MGI:2384297)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_016402. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_016402_01	euxassay_016402_02	euxassay_016402_03	euxassay_016402_04

euxassay_016402_05	euxassay_016402_06	euxassay_016402_07	euxassay_016402_08	euxassay_016402_09

euxassay_016402_10	euxassay_016402_11	euxassay_016402_12	euxassay_016402_13	euxassay_016402_14

euxassay_016402_15	euxassay_016402_16	euxassay_016402_17	euxassay_016402_18	euxassay_016402_19

euxassay_016402_20	euxassay_016402_21	euxassay_016402_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6559_wholemount_strong.wlz

6559_wholemount_moderate.wlz

6559_wholemount_weak.wlz

6559_wholemount_possible.wlz

6559_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6559_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

facial vii ganglion

moderate

single cell

moderate expression: see section 04 05 18 19

glossopharyngeal ix ganglion

moderate

single cell

moderate expression: see section 06 07 16 17

trigeminal v ganglion

moderate

single cell

moderate expression: see section 03 04 05 06 07 08 15 16 17 18 19 20

trigeminal v nerve

moderate

single cell

moderate expression: see section 08

dorsal root ganglion

moderate

single cell

moderate expression: see section 07 08 09 10 11 12 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T63122
Entity Detected:	Mtap7d1, microtubule-associated protein 7 domain containing 1 ( MGI:2384297)
Sequence:	sense strand is shown >T63122 CATCCTCTCCCTCTACAACCTGGCACAGACCTGCCTCCCCCTGCCCCAGCCCAGGACCAGGACATGCCCT TCCTCCAAAACCACCATCCCCCCGAGGCACCACTGCATCACCCAAGGGGCGGGTTCGGAGGAAGGAGGAG GCAAAAGAGAGCCCCAGCCCGTCGGGGCCTGAGGACAAAAACCACCGCAAGAGTAGAGCCGCTGAGGAGA AGGAGCCAGCTGCCCCAGCCTCACCAGCGCCCTCACCGGTGCCCTCGCCCACCCCAGCCCAGCCTCAGAA GGAGCAATCCTCAACACAGATCCCTGCAGAAACTGCTGTCCCGGCTGTCCCAGCTGCTCCGACTGCCCCT CCTACCGCTGCTCCCTCGGTGACCCCTAGCAAACCCATGGCGGGCACCACAGACCGAGAGGAAGCCACGC GGCTCCTGGCTGAAAAGCGGCGCCAGGCTCGGGAACAGAGGGAACGCGAAGAACAGGAGCGGAAGCTGCA GGCTGAAAGGGACAAGCGAATGCGAGAGGAGCAGCTGGCCCGGGAGGCTGAAGCCCGGGCTGAACGTGAG GCCGAGGCGCGGAGACGGGAGGAGCAGGAGGCTCGAGAGAAGGCGCAGGCTGAGCAGGAGGAGCAGGAGC GACTGCAGAAGCAGAAAGAGGAAGCCGAAGCCCGGTCCCGGGAAGAAGCTGAGCGCCAGCGCCAGGAGCG GGAAAAGCACTTTCAGAAGGAGGAACA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 143115. Forward Primer - name:143115_F_cDNA_BC019977, sequence:CATCCTCTCCCTCTACAACCTG; Reverse Primer - name:143115_N_SP6_cDNA_BC019977, sequence:TGTTCCTCCTTCTGAAAGTGCT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6560 same embryo
	EurExpress:euxassay_016402 same experiment
	MGI:4826485 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source