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EMAGE:6560

Casp3 caspase 3 ( MGI:107739)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:6560 EMAGE:6560 EMAGE:6560 EMAGE:6560 EMAGE:6560
"Pseudo-wholemount" of euxassay_016381. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_016381_01 euxassay_016381_02 euxassay_016381_03 euxassay_016381_04
EMAGE:6560 EMAGE:6560 EMAGE:6560 EMAGE:6560 EMAGE:6560
euxassay_016381_05 euxassay_016381_06 euxassay_016381_07 euxassay_016381_08 euxassay_016381_09
EMAGE:6560 EMAGE:6560 EMAGE:6560 EMAGE:6560 EMAGE:6560
euxassay_016381_10 euxassay_016381_11 euxassay_016381_12 euxassay_016381_13 euxassay_016381_14
EMAGE:6560 EMAGE:6560 EMAGE:6560 EMAGE:6560 EMAGE:6560
euxassay_016381_15 euxassay_016381_16 euxassay_016381_17 euxassay_016381_18 euxassay_016381_19
EMAGE:6560 EMAGE:6560
euxassay_016381_20 euxassay_016381_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:6560Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
6560_wholemount_strong.wlz
6560_wholemount_moderate.wlz
6560_wholemount_weak.wlz
6560_wholemount_possible.wlz
6560_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:6560_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
cerebral cortex mantle layer
weak weak
regionalweak expression: see section 03 04 05 06 07 08 10 11 14 15 17 18 19 20
nasal cavity olfactory epithelium
weak weak
regionalweak expression: see section 08 09 10 11 13 14
vomeronasal organ
weak weak
regionalweak expression: see section 10
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T63101
Entity Detected:Casp3, caspase 3 ( MGI:107739)
Sequence:sense strand is shown

>T63101
GTGGACTCTGGGATCTATCTGGACAGTAGTTACAAAATGGATTATCCTGAAATGGGCATATGCATAATAA
TTAATAATAAGAACTTCCATAAGAGCACTGGAATGTCATCTCGCTCTGGTACGGATGTGGACGCAGCCAA
CCTCAGAGAGACATTCATGGGCCTGAAATACCAAGTCAGGAATAAAAATGATCTTACTCGTGAAGACATT
TTGGAATTAATGGATAGTGTTTCTAAGGAAGATCATAGCAAAAGGAGCAGCTTTGTGTGTGTGATTCTAA
GCCATGGTGATGAAGGGGTCATTTATGGGACAAATGGGCCTGTTGAACTGAAAAAGTTGACTAGCTTCTT
CAGAGGCGACTACTGCCGGAGTCTGACTGGAAAGCCGAAACTCTTCATCATTCAGGCCTGCCGGGGTACG
GAGCTGGACTGTGGCATTGAGACAGACAGTGGGACTGATGAGGAGATGGCTTGCCAGAAGATACCGGTGG
AGGCTGACTTCCTGTATGCTTACTCTACAGCACCTGGTTACTATTCCTGGAGAAATTCAAAGGACGGGTC
GTGGTTCATCCAGTCCCTTTGCAGCATGCTGAAGCTGTACGCGCACAAGCTAGAATTTATGCACATTCTC
ACTCGCGTTAACAGGAAGGTGGCAACGGAATTCGAGTCCTTCTCCCTGGACTCCACTTTCCACGCAAAGA
AACAGATCCCGTGTATTGTGTCCATGCTCACGAAAGAACTGTACTTTTATCACTAGAGGAATGATTGGGG
GTGGGGGGGGGCGTGTTTCTGTTTTGTTATGC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 145306. Forward Primer - name:145306_F_cDNA_Casp3, sequence:GTGGACTCTGGGATCTATCTGG; Reverse Primer - name:145306_N_SP6_cDNA_Casp3, sequence:GCATAACAAAACAGAAACACGCC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:6559 same embryo
 EurExpress:euxassay_016381 same experiment
 MGI:4823641 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS