EMAGE mouse gene expression spatial database

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EMAGE:6567

Mir672 microRNA 672 ( MGI:3629959)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019054. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019054_01	euxassay_019054_02	euxassay_019054_03	euxassay_019054_04

euxassay_019054_05	euxassay_019054_06	euxassay_019054_07	euxassay_019054_08	euxassay_019054_09

euxassay_019054_10	euxassay_019054_11	euxassay_019054_12	euxassay_019054_13	euxassay_019054_14

euxassay_019054_15	euxassay_019054_16	euxassay_019054_17	euxassay_019054_18	euxassay_019054_19

euxassay_019054_20	euxassay_019054_21	euxassay_019054_22	euxassay_019054_23	euxassay_019054_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6567_wholemount_strong.wlz

6567_wholemount_moderate.wlz

6567_wholemount_weak.wlz

6567_wholemount_possible.wlz

6567_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6567_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 08 09 10 11 12 14 weak expression: see section 15 16

cerebral cortex mantle layer

weak

regional

weak expression: see section 09 10 11 12 14 15 16 17

olfactory cortex mantle layer

moderate

regional

moderate expression: see section 09 10 14 weak expression: see section 11 15 16

rest of cerebellum mantle layer

weak

regional

weak expression: see section 06 07 08 09 10 11 14 17

midbrain mantle layer

moderate

regional

moderate expression: see section 07 08 09 10 14 weak expression: see section 11 15 16 17 18

trigeminal v ganglion

weak

single cell

weak expression: see section 05 06 09 17 19 20

dorsal grey horn

weak

regional

weak expression: see section 11 13 14 15

ventral grey horn

weak

regional

weak expression: see section 11 12 13 15 16

dorsal root ganglion

moderate

single cell

moderate expression: see section 09 10 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T70119
Entity Detected:	Mir672, microRNA 672 ( MGI:3629959)
Sequence:	sense strand is shown >T70119 TGAGGTTGGTGTACTGTGTGTGA
Notes:	The probe used by the EURExpress consortium to detect the presence of miRNA mmu-miR-672 was custom designed and ordered from Sigma-Exiqon by the EURExpress partner in Geneva and then distributed to the ISH generating units within the consortium.
Chemistry:	LNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	MGI:4826355 same experiment
	EurExpress:euxassay_019054 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source