EMAGE mouse gene expression spatial database

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EMAGE:6569

Mir679 microRNA 679 ( MGI:3629899)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019062. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019062_01	euxassay_019062_02	euxassay_019062_03	euxassay_019062_04

euxassay_019062_05	euxassay_019062_06	euxassay_019062_07	euxassay_019062_08	euxassay_019062_09

euxassay_019062_10	euxassay_019062_11	euxassay_019062_12	euxassay_019062_13	euxassay_019062_14

euxassay_019062_15	euxassay_019062_16	euxassay_019062_17	euxassay_019062_18	euxassay_019062_19

euxassay_019062_20	euxassay_019062_21	euxassay_019062_22	euxassay_019062_23	euxassay_019062_24

euxassay_019062_25	euxassay_019062_26

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6569_wholemount_strong.wlz

6569_wholemount_moderate.wlz

6569_wholemount_weak.wlz

6569_wholemount_possible.wlz

6569_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6569_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18

telencephalon

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25

hindbrain

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

midbrain

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

facial vii ganglion

moderate

regional

moderate expression: see section 05 06 21 22 weak expression: see section 04

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 18 19 20 weak expression: see section 07 08

trigeminal v ganglion

moderate

regional

moderate expression: see section 05 06 17 18 19 20 21 22 23 weak expression: see section 03 04 07 08

spinal cord

moderate

regional

moderate expression: see section 11 12 13 14 15 16 17 18 19

dorsal root ganglion

moderate

regional

moderate expression: see section 08 09 10 14 15 16 17 18 19 20 weak expression: see section 11 12 13

neural retina

moderate

regional

moderate expression: see section 01 weak expression: see section 02 23 24 25

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T70128
Entity Detected:	Mir679, microRNA 679 ( MGI:3629899)
Sequence:	sense strand is shown >T70128 GGACTGTGAGGTGACTCTTGGT
Notes:	The probe used by the EURExpress consortium to detect the presence of miRNA mmu-miR-679 was custom designed and ordered from Sigma-Exiqon by the EURExpress partner in Geneva and then distributed to the ISH generating units within the consortium.
Chemistry:	LNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6568 same embryo
	EMAGE:6570 same embryo
	EurExpress:euxassay_019062 same experiment
	MGI:4826360 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source