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EMAGE:657

Fgf4 fibroblast growth factor 4 ( MGI:95518)
TS14 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:657 EMAGE:657
Fig 4E of Niswander & Martin, 1992 [PMID:1618140] . Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright. Fig 4D of Niswander & Martin, 1992 [PMID:1618140] . Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Corresponding bright and darkfield images shown below. Abbreviations used: be, branchial arch; nt, neural tube; phe, pharyngeal endoderm; 1 ba, 1st branchial arch; 2 ba, 2nd branchial arch; *, foregut.
Expression Pattern Description
Spatial Annotation:
EMAGE:657EMAGE:657Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D context movie

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
657_voxel_strong_3D_1.wlz
657_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:657_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
1st branchial arch mandibular component ectoderm
detected detected
regionalFgf-4 RNA is found in surface ectoderm covering the anterior half of the 1st (mandibular) and 2nd (hyoid) branchial arches.
pharyngeal region
not detected not detected
2nd branchial arch ectoderm
detected detected
regionalFgf-4 RNA is found in surface ectoderm covering the anterior half of the 1st (mandibular) and 2nd (hyoid) branchial arches.
1st branchial pouch endoderm
detected detected
regionalFgf-4 RNA is found in surface ectoderm covering the anterior half of the 1st (mandibular) and 2nd (hyoid) branchial arches.
1st branchial pouch
detected detected
regionalFgf-4 is not expressed in the medial aspects of the pouch
future brain
not detected not detected
2nd branchial pouch
detected detected
regionalFgf-4 is not expressed in the medial aspects of the pouch.
2nd branchial pouch endoderm
detected detected
regionalFgf-4 is not expressed in the medial aspects of the pouch
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:17265
Entity Detected:Fgf4, fibroblast growth factor 4 ( MGI:95518)
Notes:Two Fgf4 probes were used by Niswander et al, 1992 [PMID:1618140] , each giving "identical results": the "full length coding sequence (see Hebert et al, 1990 [PMID:2318343] " and "the 3' untranslated region from nt 3630-3819 (see Brookes et al, 1989 [PMID:2740210] ". Unable to ascertain which was used here.
Chemistry:RNA
Strand:antisense
Label:S35
Specimen
Organism:mouse
Strain:Swiss Webster
Age:9.5 dpc
Theiler Stage:TS14
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Embedding:paraffin
Staining procedure:autoradiography
General Information
Authors:Niswander & Martin, 1992 [PMID:1618140] . Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:1618140] Niswander L, Martin GR 1992 Fgf-4 expression during gastrulation, myogenesis, limb and tooth development in the mouse. Development (114):755-68
 [ doi:10.1016/0012-1606(90)90211-Z] [ PMID:2318343] H�bert JM, Basilico C, Goldfarb M, Haub O, Martin GR 1990 Isolation of cDNAs encoding four mouse FGF family members and characterization of their expression patterns during embryogenesis. Dev Biol (138):454-63
 [ doi:10.1093/nar/17.11.4037] [ PMID:2740210] Brookes S, Smith R, Thurlow J, Dickson C, Peters G 1989 The mouse homologue of hst/k-FGF: sequence, genome organization and location relative to int-2. Nucleic Acids Res (17):4037-45
Links:MGI:1331200 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI