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EMAGE:6571

Mir574 microRNA 574 ( MGI:3718549)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:6571 EMAGE:6571 EMAGE:6571 EMAGE:6571 EMAGE:6571
"Pseudo-wholemount" of euxassay_019039. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_019039_01 euxassay_019039_02 euxassay_019039_03 euxassay_019039_04
EMAGE:6571 EMAGE:6571 EMAGE:6571 EMAGE:6571 EMAGE:6571
euxassay_019039_05 euxassay_019039_06 euxassay_019039_07 euxassay_019039_08 euxassay_019039_09
EMAGE:6571 EMAGE:6571 EMAGE:6571 EMAGE:6571 EMAGE:6571
euxassay_019039_10 euxassay_019039_11 euxassay_019039_12 euxassay_019039_13 euxassay_019039_14
EMAGE:6571 EMAGE:6571 EMAGE:6571 EMAGE:6571 EMAGE:6571
euxassay_019039_15 euxassay_019039_16 euxassay_019039_17 euxassay_019039_18 euxassay_019039_19
EMAGE:6571 EMAGE:6571 EMAGE:6571 EMAGE:6571 EMAGE:6571
euxassay_019039_20 euxassay_019039_21 euxassay_019039_22 euxassay_019039_23 euxassay_019039_24
EMAGE:6571
euxassay_019039_25

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:6571Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
6571_wholemount_strong.wlz
6571_wholemount_moderate.wlz
6571_wholemount_weak.wlz
6571_wholemount_possible.wlz
6571_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:6571_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
diencephalon lateral wall mantle layer
weak weak
regionalweak expression: see section 08 09 10 11 12 13 14 15 16 17
cerebral cortex mantle layer
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 16 17 18 19 20 21 22 23 24
telencephalon mantle layer
moderate moderate
regionalmoderate expression: see section 05 06 19 20 22 23
olfactory cortex mantle layer
moderate moderate
regionalmoderate expression: see section 10 11 12 16 17 18
rest of cerebellum mantle layer
moderate moderate
regionalmoderate expression: see section 05 06 07 11 13 14 15 16 17 18 19 20 21 weak expression: see section 04 09 10
midbrain mantle layer
moderate moderate
regionalmoderate expression: see section 06 07 08 10 11 12 13 14 15 16 17 18 weak expression: see section 09
facial vii ganglion
moderate moderate
regionalmoderate expression: see section 05 20 21 22
glossopharyngeal ix ganglion
moderate moderate
regionalmoderate expression: see section 09 19 weak expression: see section 07 08 18
trigeminal v ganglion
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 09 17 18 19 20 21 22
dorsal grey horn
moderate moderate
regionalmoderate expression: see section 10 11 13 14
ventral grey horn
moderate moderate
regionalmoderate expression: see section 11 12 13 14 15
dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 07 08 09 10 11 12 15 16 17 18
neural retina
moderate moderate
regionalmoderate expression: see section 24 weak expression: see section 01 02 03 04 25
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T70094
Entity Detected:Mir574, microRNA 574 ( MGI:3718549)
Sequence:sense strand is shown

>T70094
CACGCTCATGCACACACCCACA
Notes:The probe used by the EURExpress consortium to detect the presence of miRNA mmu-miR-574-3p was custom designed and ordered from Sigma-Exiqon by the EURExpress partner in Geneva and then distributed to the ISH generating units within the consortium.
Chemistry:LNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:6573 same embryo
 EMAGE:6572 same embryo
 EurExpress:euxassay_019039 same experiment
 MGI:4826332 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS