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EMAGE:658

Wnt7b wingless-related MMTV integration site 7B ( MGI:98962)
TS16 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:658 EMAGE:658
Figure 6G of Liu et al., 1999 [PMID:10518499] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright. Figure 6A of Liu et al., 1999 [PMID:10518499] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: no stars
Notes:
Roughly equivalent sections for bright- and dark-field are shown below. Image annotations: Fig 6G: red arrowhead indicates the caudal boundary of expression. Fig 6A: Tel - telencephalon; Di - diencephalon; Mes - mesencephalon; Met - metencephalon; Mye - myelencephalon.
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
telencephalon
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:9296
Entity Detected:Wnt7b, wingless-related MMTV integration site 7B ( MGI:98962)
Sequence:sense strand is shown

>MGI:9296
AGGCACAGGAGGGTGGGGATAGAGGTGGGGAGCTCGAGATACTCCAGGCTCCTTCCTACTCGCTCTGTCC
CCGCCCAGCATCCAAGGTCAACGCAATGGTGGTCTGGTACCCAATGGAGACAAATCCCTTTACTTCTCTT
TGGGAAAGTGAACCACAAAGGGACCATGAGACTCTGAGGGTCACCTCCCTGCCTGTGACTGGACACAGAA
AGGCCACACCCACCAGTCACACTCAAAACGGTTTCCTGGGCTGTTTCCTGCCGGCCCTGGGCAGTGTGGA
TGGATGTTGACAAAATTATTTATGTTT
nt 1143 - nt 1449 of NM_009528.1
Notes:The probe used in this study by Liu et al., 1999 [PMID:10518499] , was previously described by Parr et al., 1993 [PMID:8275860] as a "306bp PstI-EcoRI (3' untranslated)".
Chemistry:RNA
Strand:antisense
Label:S35
Specimen
Organism:mouse
Strain:Swiss
Age:9.5 dpc
Theiler Stage:TS16
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Embedding:cryosection
Staining procedure:autoradiography
General Information
Authors:Liu et al., 1999 [PMID:10518499] Indexed by GXD.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:10518499] Liu A, Losos K, Joyner AL 1999 FGF8 can activate Gbx2 and transform regions of the rostral mouse brain into a hindbrain fate. Development (126):4827-38
 [ PMID:8275860] Parr BA, Shea MJ, Vassileva G, McMahon AP 1993 Mouse Wnt genes exhibit discrete domains of expression in the early embryonic CNS and limb buds. Development (119):247-61
Links:MGI:1349188 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI