EMAGE mouse gene expression spatial database

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EMAGE:6586

Mir669a-1 microRNA 669a-1 ( MGI:3629611)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019048. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019048_01	euxassay_019048_02	euxassay_019048_03	euxassay_019048_04

euxassay_019048_05	euxassay_019048_06	euxassay_019048_07	euxassay_019048_08	euxassay_019048_09

euxassay_019048_10	euxassay_019048_11	euxassay_019048_12	euxassay_019048_13	euxassay_019048_14

euxassay_019048_15	euxassay_019048_16	euxassay_019048_17	euxassay_019048_18	euxassay_019048_19

euxassay_019048_20	euxassay_019048_21	euxassay_019048_22	euxassay_019048_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6586_wholemount_strong.wlz

6586_wholemount_moderate.wlz

6586_wholemount_weak.wlz

6586_wholemount_possible.wlz

6586_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6586_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 10 11 13 14 15 16 17 weak expression: see section 08 09 12

cerebral cortex mantle layer

moderate

regional

moderate expression: see section 05 06 13 14 17 21 22 weak expression: see section 07 08 10 11 12 18 19 20

telencephalon mantle layer

moderate

regional

moderate expression: see section 05 06 10 16 21 22 23 weak expression: see section 07 11 18 19 20

olfactory cortex mantle layer

moderate

regional

moderate expression: see section 10 14 15 16 weak expression: see section 09 11

medulla oblongata alar plate mantle layer

moderate

regional

moderate expression: see section 13 16 17 weak expression: see section 08 09 10 11 12 14 15

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 13 16 17 weak expression: see section 09 10 11 12 14 15

rest of cerebellum mantle layer

moderate

regional

moderate expression: see section 16 17 20 21 weak expression: see section 05 06 07 08 18 19

pons mantle layer

moderate

regional

moderate expression: see section 13 16 17 weak expression: see section 07 08 09 10 11 12 14 15 18

midbrain mantle layer

moderate

regional

moderate expression: see section 06 07 08 10 11 12 13 14 15 16 17 18 weak expression: see section 09 19

glossopharyngeal ix ganglion

weak

single cell

weak expression: see section 17

trigeminal v ganglion

moderate

single cell

moderate expression: see section 05 06 16 17 18 19 20 21 weak expression: see section 07 09

dorsal grey horn

moderate

regional

moderate expression: see section 13 14 weak expression: see section 10 11 12

ventral grey horn

moderate

regional

moderate expression: see section 13 14 weak expression: see section 10 11 15

dorsal root ganglion

moderate

single cell

moderate expression: see section 08 09 13 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T70113
Entity Detected:	Mir669a-1, microRNA 669a-1 ( MGI:3629611)
Sequence:	sense strand is shown >T70113 AGTTGTGTGTGCATGTTCATGT
Notes:	The probe used by the EURExpress consortium to detect the presence of miRNA mmu-miR-669a was custom designed and ordered from Sigma-Exiqon by the EURExpress partner in Geneva and then distributed to the ISH generating units within the consortium.
Chemistry:	LNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6595 same assay
	EurExpress:euxassay_019048 same experiment
	EMAGE:6597 same assay
	EMAGE:6598 same assay
	EMAGE:6587 same assay
	EMAGE:6588 same assay
	EMAGE:6589 same assay
	EMAGE:6590 same assay
	EMAGE:6591 same assay
	EMAGE:6592 same assay
	EMAGE:6593 same assay
	EMAGE:6594 same assay
	EMAGE:6585 same embryo
	MGI:4826345 same experiment
	MGI:4826346 same experiment
	MGI:4826351 same experiment
	MGI:4826352 same experiment
	MGI:4826344 same experiment
	MGI:4826343 same experiment
	MGI:4826341 same experiment
	MGI:4826350 same experiment
	MGI:4826342 same experiment
	MGI:4826347 same experiment
	MGI:4826348 same experiment
	MGI:4826349 same experiment
	MGI:4826340 same experiment
	EMAGE:6596 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source