EMAGE mouse gene expression spatial database

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EMAGE:6614

Mir671 microRNA 671 ( MGI:3629656)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019053. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019053_01	euxassay_019053_02	euxassay_019053_03	euxassay_019053_04

euxassay_019053_05	euxassay_019053_06	euxassay_019053_07	euxassay_019053_08	euxassay_019053_09

euxassay_019053_10	euxassay_019053_11	euxassay_019053_12	euxassay_019053_13	euxassay_019053_14

euxassay_019053_15	euxassay_019053_16	euxassay_019053_17	euxassay_019053_18	euxassay_019053_19

euxassay_019053_20	euxassay_019053_21	euxassay_019053_22	euxassay_019053_23	euxassay_019053_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6614_wholemount_strong.wlz

6614_wholemount_moderate.wlz

6614_wholemount_weak.wlz

6614_wholemount_possible.wlz

6614_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6614_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

weak

regional

weak expression: see section 02 03 06 07 08 09 19 20 21 22 23 24

humerus

weak

regional

weak expression: see section 01 02 03 23 24

hindlimb digit 2 metatarsal

weak

regional

weak expression: see section 22

hindlimb digit 3 metatarsal

weak

regional

weak expression: see section 22

hindlimb digit 4 metatarsal

weak

regional

weak expression: see section 22

foot mesenchyme

weak

regional

weak expression: see section 21

fibula

weak

regional

weak expression: see section 22 23 24

tibia

weak

regional

weak expression: see section 23

femur

weak

regional

weak expression: see section 02 03 04 19 20 21 22

neural retina

moderate

regional

moderate expression: see section 01 weak expression: see section 02 21 22 23

meckel's cartilage

weak

regional

weak expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

axial skeleton

weak

regional

weak expression: see section 10 11 12 13 19

clavicle

weak

regional

weak expression: see section 09 10 17 18

scapula

weak

regional

weak expression: see section 04 05 06 22 23 24

pelvic girdle skeleton

weak

regional

weak expression: see section 09 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T70118
Entity Detected:	Mir671, microRNA 671 ( MGI:3629656)
Sequence:	sense strand is shown >T70118 AGGAAGCCCTGGAGGGGCTGGAG
Notes:	The probe used by the EURExpress consortium to detect the presence of miRNA mmu-miR-671-5p was custom designed and ordered from Sigma-Exiqon by the EURExpress partner in Geneva and then distributed to the ISH generating units within the consortium.
Chemistry:	LNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	MGI:4826354 same experiment
	EurExpress:euxassay_019053 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source