EMAGE mouse gene expression spatial database

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EMAGE:6623

Mir218-1 microRNA 218-1 ( MGI:3618751)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_019036. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_019036_01	euxassay_019036_02	euxassay_019036_03	euxassay_019036_04

euxassay_019036_05	euxassay_019036_06	euxassay_019036_07	euxassay_019036_08	euxassay_019036_09

euxassay_019036_10	euxassay_019036_11	euxassay_019036_12	euxassay_019036_13	euxassay_019036_14

euxassay_019036_15	euxassay_019036_16	euxassay_019036_17	euxassay_019036_18	euxassay_019036_19

euxassay_019036_20	euxassay_019036_21	euxassay_019036_22	euxassay_019036_23	euxassay_019036_24

euxassay_019036_25	euxassay_019036_26

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6623_wholemount_strong.wlz

6623_wholemount_moderate.wlz

6623_wholemount_weak.wlz

6623_wholemount_possible.wlz

6623_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6623_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 09 17 18 19 weak expression: see section 05 06 07 08 10 11 12 20 21 22 23 24 25 26

humerus

moderate

regional

moderate expression: see section 01 weak expression: see section 02 03 04 05 24 25 26

fibula

weak

regional

weak expression: see section 02 03 04

tibia

weak

regional

weak expression: see section 02 03

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 16 17 moderate expression: see section 09 10

pons mantle layer

moderate

regional

moderate expression: see section 19 weak expression: see section 08 09 18

midbrain mantle layer

moderate

regional

moderate expression: see section 12 14 15 weak expression: see section 13

abducent vi nerve

weak

regional

weak expression: see section 12 15

ventral grey horn

strong

regional

strong expression: see section 13 14 15 16 17 moderate expression: see section 11 12

otic capsule

moderate

regional

moderate expression: see section 10 17 18 weak expression: see section 09

hyoid

moderate

regional

moderate expression: see section 17 weak expression: see section 11 12 13 14 15

meckel's cartilage

moderate

regional

moderate expression: see section 05 09 10 17 18 weak expression: see section 04 06 07 08 11 12 13 14 16 19 20 21 22 23 24

axial skeleton

moderate

regional

moderate expression: see section 09 17 18 19 weak expression: see section 10 11 12 13 14 15 16

basisphenoid bone

weak

regional

weak expression: see section 10 11 12 13 14 15 16 17 19

orbito-sphenoid

weak

regional

weak expression: see section 09 10 11 12 13 14 15 17

viscerocranium

weak

regional

Expression in the turbinate bone.

scapula

weak

regional

weak expression: see section 02 03 04 05 23 24 25

pelvic girdle skeleton

weak

regional

weak expression: see section 11 12 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T70343
Entity Detected:	Mir218-1, microRNA 218-1 ( MGI:3618751)
Sequence:	sense strand is shown >T70343 TTGTGCTTGATCTAACCATGT
Notes:	The probe used by the EURExpress consortium to detect the presence of miRNA mmu-miR-218 was custom designed and ordered from Sigma-Exiqon by the EURExpress partner in Geneva and then distributed to the ISH generating units within the consortium.
Chemistry:	LNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6622 same assay
	EurExpress:euxassay_019036 same experiment
	EMAGE:6621 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source