EMAGE mouse gene expression spatial database

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EMAGE:6627

Ube2d1 ubiquitin-conjugating enzyme E2D 1, UBC4/5 homolog (yeast) ( MGI:2384911)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002231. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002231_01	euxassay_002231_02	euxassay_002231_03	euxassay_002231_04

euxassay_002231_05	euxassay_002231_06	euxassay_002231_07	euxassay_002231_08	euxassay_002231_09

euxassay_002231_10	euxassay_002231_11	euxassay_002231_12	euxassay_002231_13	euxassay_002231_14

euxassay_002231_15	euxassay_002231_16	euxassay_002231_17	euxassay_002231_18	euxassay_002231_19

euxassay_002231_20	euxassay_002231_21	euxassay_002231_22	euxassay_002231_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6627_wholemount_strong.wlz

6627_wholemount_moderate.wlz

6627_wholemount_weak.wlz

6627_wholemount_possible.wlz

6627_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6627_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 weak expression: see section 23

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 08 19

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 17 18 19 weak expression: see section 20 21 22

spinal cord

moderate

homogeneous

moderate expression: see section 10 11 12 13 14 15 16 17 18

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 10 17

cervical ganglion

moderate

regional

moderate expression: see section 09 18

thoracic ganglion

moderate

regional

moderate expression: see section 12 14

dorsal root ganglion

moderate

regional

moderate expression: see section 09 10 14 15 16 17 18 19

urethra of male

moderate

regional

moderate expression: see section 12 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2039
Entity Detected:	Ube2d1, ubiquitin-conjugating enzyme E2D 1, UBC4/5 homolog (yeast) ( MGI:2384911)
Sequence:	sense strand is shown >T2039 GGCACTCCTGACTCCTCCTCCCCACCCCCATCCCCCGTGAACAGTATTGAAGCTCCCAACAGCTGATGAT GAATTAACTGCCCCCGCCCCTTGTCTTAGCATATTCTGTGGGTTGAATGCGTATTTCTTAAACCGGAACT GATCTGCACACTGTATTTCTCAGTATACTGATTAGAATGGAGAAACACTTGTTTGGGTTTTATTGTACTT GACTTAACTTTATTGCAATGTGAATTAATTGCACTGTTAGGTAGGAAGGTGTGTGACTTTTTTTTGTTGT TGCTCTTCACCTTTGACCTTTTTTTTTTTCTGTATAGGCAATATTATATTGACACCTTTTACAGATCTGA CTGTAGCCTTTTCCATATAAATAAAATGCTTTTTCTACTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:752516 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:752516 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6625 same embryo
	EMAGE:6624 same embryo
	EMAGE:6626 same embryo
	EurExpress:euxassay_002231 same experiment
	MGI:4829051 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source