EMAGE mouse gene expression spatial database

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EMAGE:6653

Igsf8 immunoglobulin superfamily, member 8 ( MGI:2154090)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002218. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002218_01	euxassay_002218_02	euxassay_002218_03	euxassay_002218_04

euxassay_002218_05	euxassay_002218_06	euxassay_002218_07	euxassay_002218_08	euxassay_002218_09

euxassay_002218_10	euxassay_002218_11	euxassay_002218_12	euxassay_002218_13	euxassay_002218_14

euxassay_002218_15	euxassay_002218_16	euxassay_002218_17	euxassay_002218_18	euxassay_002218_19

euxassay_002218_20	euxassay_002218_21	euxassay_002218_22	euxassay_002218_23	euxassay_002218_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6653_wholemount_strong.wlz

6653_wholemount_moderate.wlz

6653_wholemount_weak.wlz

6653_wholemount_possible.wlz

6653_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6653_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus ventricular layer

moderate

regional

moderate expression: see section 10 11 12 13 14

diencephalon lateral wall ventricular layer

moderate

regional

moderate expression: see section 11 12 13 14

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

rest of cerebellum ventricular layer

moderate

regional

moderate expression: see section 04 05 06 16 17 19 20

pons ventricular layer

moderate

regional

moderate expression: see section 08 09 14 15 16

midbrain ventricular layer

strong

regional

strong expression: see section 08 moderate expression: see section 09 10 11 12 13 14 15 16 17 18

nasal cavity epithelium

moderate

regional

moderate expression: see section 17

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 10 13 14 15 17

meckel's cartilage

moderate

regional

moderate expression: see section 03 04 05 07 08 weak expression: see section 09 10 15 16 17 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2977
Entity Detected:	Igsf8, immunoglobulin superfamily, member 8 ( MGI:2154090)
Sequence:	sense strand is shown >T2977 TGGCCTCGAGCCAGATTCGGACGAGGCGGATTGGCCCAGGCGAACCCTTAGAACTGCTGTGCAATGTGTC CGGTGCACTGCCCCCACCAGGCCGTCATGCTGCGTACTCTGTGGGCTGGGAGATGGCCCCTGCAGGGGCT CCTGGACCCGGCCGCCTGGTGGCCCAGCTGGACACGGAAGGTATAGGCAGCCTGGGCCCTGGCTATGAGG ACCGGCACATTGCCATGGAGAAAGTAGCATCCAGAACCTACCGACTGCGGCTGGAGGCTGCCAGGCCCGC TGATGCAGGCACCTACCGCTGCCTCGCCAAAGCCTATGTTCGAGGGTCTGGGACCCGGCTTCGTGAAGCG GCCAGTGCTCGTTCCCGGCCTCTCCCTGTGCATGTGAGAGAAGAAGGCGTGGTGCTAGAGGCTGTGGCGT GGCTAGCAGGGGGCACTGTGTACCGGGGAGAAACGGCCTCCCTGCTATGCAACATATCTGTGCGGGGCGG CCCCCCAGGGCTGCGACTAGCAGCCAGCTGGTGGGTGGAGAGGCCAGAGGAGGGCGA
Notes:	The probe template was PCR amplified from IMAGE:1532939 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1532939 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6652 same embryo
	EMAGE:6651 same embryo
	EMAGE:6655 same embryo
	EMAGE:6654 same embryo
	EMAGE:6650 same embryo
	EurExpress:euxassay_002218 same experiment
	MGI:4825548 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source