EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:6679

Ppp1r14b protein phosphatase 1, regulatory (inhibitor) subunit 14B ( MGI:107682)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002549. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002549_01	euxassay_002549_02	euxassay_002549_03	euxassay_002549_04

euxassay_002549_05	euxassay_002549_06	euxassay_002549_07	euxassay_002549_08	euxassay_002549_09

euxassay_002549_10	euxassay_002549_11	euxassay_002549_12	euxassay_002549_13	euxassay_002549_14

euxassay_002549_15	euxassay_002549_16	euxassay_002549_17	euxassay_002549_18	euxassay_002549_19

euxassay_002549_20	euxassay_002549_21	euxassay_002549_22	euxassay_002549_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6679_wholemount_strong.wlz

6679_wholemount_moderate.wlz

6679_wholemount_weak.wlz

6679_wholemount_possible.wlz

6679_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6679_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

femur

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 23 weak expression: see section 17 21 22

nucleus pulposus

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 19

cranium

weak

regional

weak expression: see section 02 03 04 05 06 07 08 09 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3390
Entity Detected:	Ppp1r14b, protein phosphatase 1, regulatory (inhibitor) subunit 14B ( MGI:107682)
Sequence:	sense strand is shown >T3390 TGGCCTCGAGNCAGATTCGGACGAGGTGCGCCGCAGAGTCAATATAAAAACACAAAAAGTCCCGTCAGTT TAATAACAATAAAAAACCCCAAAAGTGGAAAACTGAGGGCAAGGGAAATGACCCCTAGGCAGGGGCACGG GGATCCCTGCTCATGGCACCAGGCCTGGCTGCAGGGTCCCCCGGTATTGCTGTTGCTACGAGGTTGGGGG GCAGCGATTGTCCTGTGGGAGCCACGGCCGTCTGAGTCAGGACCCTCATTTCTTCTGGGGTGTGCTCAGC TTCTGCATGCCCCGGATCTTGTCCAGCAGGCCAGAGATGAAGGCCTCAGTGGGTTTGTAACAGTCAACTA ACAGCTCCTTGACCCTAGCAGCCCGGGTATCATCACTCTCCATGTCTAAAAGTTCATCCACATCTATCTC GAGCTCCGGGATCTCCTCCTCCTGGCAGTCGTAGAGTCGCGTGAGCTGTTCTAAAATCCACTCCTCCAGG TTGAGGCGCTTCCGTAGCTCCTTGCGGTCGTACTTGACGGTGACCTTCC
Notes:	The probe template was PCR amplified from IMAGE:2939167 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2939167 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6682 same embryo
	EMAGE:6681 same embryo
	EMAGE:6680 same embryo
	EurExpress:euxassay_002549 same experiment
	MGI:4827370 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source