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EMAGE:6687

Cobra1 cofactor of BRCA1 ( MGI:1931035)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:6687 EMAGE:6687 EMAGE:6687 EMAGE:6687 EMAGE:6687
"Pseudo-wholemount" of euxassay_003273. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_003273_01 euxassay_003273_02 euxassay_003273_03 euxassay_003273_04
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euxassay_003273_15 euxassay_003273_16 euxassay_003273_17 euxassay_003273_18 euxassay_003273_19
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euxassay_003273_20 euxassay_003273_21 euxassay_003273_22 euxassay_003273_23 euxassay_003273_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:6687Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
6687_wholemount_strong.wlz
6687_wholemount_moderate.wlz
6687_wholemount_weak.wlz
6687_wholemount_possible.wlz
6687_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:6687_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T3315
Entity Detected:Cobra1, cofactor of BRCA1 ( MGI:1931035)
Sequence:sense strand is shown

>T3315
GTGCACCGTCATGTGCTGAGGCTTCTCCTCCACTTGCATGCAAGAGTGGCCCCTTCCAAGCTGGAGGCAT
TGCAGAAGGCTCTGGAGCCCACAGGCCAGAGCGGGGAGGCCGTGAAGGAGCTCTACTCCCAGCTTGGTGA
GAAGTTGGAGCAGTTAGACCATCGAAAGCCCAGTCCTACCCAAGCTGCAGAGACACCAGCACTGGATCTG
CCTCTTCCCAGCGTGCCTGCTCCAGCCACACTGTGAGGCTGTGGAGAACTGAAACTGTTTGACAGTGGTG
CCCTATGGAAGGGCACCTAGTTGGCTAGGGGTGTAGGCTGAGCTGAGAGGGGTGTTCTTTGGCAAGGTTC
TCCCTCCTCTAGTTTCTCCCAGGAGCTGGTCCCAAAAGGTCCTCCCTCAGTGGTCTACTTGCATATCATA
GGGGGCCCTTGATGTCATGGTGGGACTTGGCCCCTGCTTGACCCTGGCAGAGGTGGATGGTGATTGGGAT
ATAGGAAAAGCAGTGGGCAGAGGCCTGACTGACCTGATAT
Notes:The probe template was PCR amplified from IMAGE:2780983 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2780983 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:6684 same embryo
 EMAGE:6686 same embryo
 EMAGE:6688 same embryo
 EMAGE:6683 same embryo
 EMAGE:6685 same embryo
 EurExpress:euxassay_003273 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS