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EMAGE:6702

Slc35a5 solute carrier family 35, member A5 ( MGI:1921352)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:6702 EMAGE:6702 EMAGE:6702 EMAGE:6702 EMAGE:6702
"Pseudo-wholemount" of euxassay_002309. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_002309_01 euxassay_002309_02 euxassay_002309_03 euxassay_002309_04
EMAGE:6702 EMAGE:6702 EMAGE:6702 EMAGE:6702 EMAGE:6702
euxassay_002309_05 euxassay_002309_06 euxassay_002309_07 euxassay_002309_08 euxassay_002309_09
EMAGE:6702 EMAGE:6702 EMAGE:6702 EMAGE:6702 EMAGE:6702
euxassay_002309_10 euxassay_002309_11 euxassay_002309_12 euxassay_002309_13 euxassay_002309_14
EMAGE:6702 EMAGE:6702 EMAGE:6702 EMAGE:6702 EMAGE:6702
euxassay_002309_15 euxassay_002309_16 euxassay_002309_17 euxassay_002309_18 euxassay_002309_19
EMAGE:6702 EMAGE:6702 EMAGE:6702 EMAGE:6702
euxassay_002309_20 euxassay_002309_21 euxassay_002309_22 euxassay_002309_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:6702Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
6702_wholemount_strong.wlz
6702_wholemount_moderate.wlz
6702_wholemount_weak.wlz
6702_wholemount_possible.wlz
6702_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:6702_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T2031
Entity Detected:Slc35a5, solute carrier family 35, member A5 ( MGI:1921352)
Sequence:sense strand is shown

>T2031
GGCCTCGAGCCAGATTCGGCACGAGGGCCATGTTATCATACTTAGCACTTGTATTTTAATTCACCTAAGC
CATTGTAGAATTGTTTGTTTGTTTGTTTGTTTGCTCTATCCTTGGTTTGTACATTTACAAAATCTTGACA
TCATTACCTTTTGATGTTGAGTTACTGTGCTGAGAATTCAGTTGATTTATTTTTGAGTTATTAATTGAGC
TGTAGAATTGTTACCTATCTTTGTGTAATATCCTATTCTGAAAGTCAAGTCTCTGGCTTTAGGATGCCTT
TATAAGAATTATTTTGTAAAAAAAAAAAATAATAATAATGACTTTTAATGAAATAGAGTAGTGTTACTCC
AAGAGGGCCAGTAACCAGAAAGTCACCTCTCAACAACACTTCCTACTTAGAAGTGCATGGTATTTCTCAA
GACAAGTTTGCATGCAGCCAGGTGATTCTTCATTGGTACAGCAGTCATGTGGCAGATAATTATAATGTAA
GAAAATAGAGTGATTTGTTCCAGGTCTTATAGCTGAAGGGTAGTAGAAACAAGCTTCAA
Notes:The probe template was PCR amplified from IMAGE:751119 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:751119 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:6700 same embryo
 EMAGE:6701 same embryo
 EMAGE:6699 same embryo
 EMAGE:6705 same embryo
 EMAGE:6704 same embryo
 EMAGE:6703 same embryo
 EurExpress:euxassay_002309 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS