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EMAGE:6707

Ube2i ubiquitin-conjugating enzyme E2I ( MGI:107365)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:6707 EMAGE:6707 EMAGE:6707 EMAGE:6707 EMAGE:6707
"Pseudo-wholemount" of euxassay_002278. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_002278_01 euxassay_002278_02 euxassay_002278_03 euxassay_002278_04
EMAGE:6707 EMAGE:6707 EMAGE:6707 EMAGE:6707 EMAGE:6707
euxassay_002278_05 euxassay_002278_06 euxassay_002278_07 euxassay_002278_08 euxassay_002278_09
EMAGE:6707 EMAGE:6707 EMAGE:6707 EMAGE:6707 EMAGE:6707
euxassay_002278_10 euxassay_002278_11 euxassay_002278_12 euxassay_002278_13 euxassay_002278_14
EMAGE:6707 EMAGE:6707 EMAGE:6707 EMAGE:6707 EMAGE:6707
euxassay_002278_15 euxassay_002278_16 euxassay_002278_17 euxassay_002278_18 euxassay_002278_19
EMAGE:6707 EMAGE:6707 EMAGE:6707 EMAGE:6707 EMAGE:6707
euxassay_002278_20 euxassay_002278_21 euxassay_002278_22 euxassay_002278_23 euxassay_002278_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:6707Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
6707_wholemount_strong.wlz
6707_wholemount_moderate.wlz
6707_wholemount_weak.wlz
6707_wholemount_possible.wlz
6707_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:6707_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T3303
Entity Detected:Ube2i, ubiquitin-conjugating enzyme E2I ( MGI:107365)
Sequence:sense strand is shown

>T3303
GCCAAAATGTAAATTTGAGCCCCCACTGTTTCATCCAAACGTGTATCCTTCTGGCACAGTGTGCCTGTCC
ATCCTGGAGGAAGACAAGGACTGGAGGCCAGCTATCACCATCAAACAGATCTTATTAGGAATACAAGAAC
TTCTAAATGAACCAAATATTCAAGACCCAGCTCAAGCAGAGGCCTACACAATTTACTGCCAAAACAGAGT
GGAATATGAGAAAAGGGTCCGAGCACAAGCGAAGAAGTTTGCCCCCTCATAAGCAGCGGCCCTGGGCTCC
ATGACGAGGAAGGGATTGGCTTGGCAAGAACTTGTTTACAACCTTTTGCAGATCTAAGTCGCTCCGTACA
GTTACTAGTCGCCTGGGAGGGTTGAGCGGGCGCCATTTTCCATTTCCGCCACTGGCATATTCAGTCTTTT
GTATTTTTGATTATTAAGTAAAACTTGCTTTTATTTTAATATTGATGTCAGTATTTCAACTGCTGTAAAG
TGATAAACTTTTGTACTTGGTAAGCCCCTAGCAGCTAGT
Notes:The probe template was PCR amplified from IMAGE:2780679 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2780679 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:6708 same embryo
 EMAGE:6709 same embryo
 EMAGE:6706 same embryo
 EMAGE:6710 same embryo
 EurExpress:euxassay_002278 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS