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EMAGE:671

Myf5 myogenic factor 5 ( MGI:97252)
TS17
in situ hybridisation

Data Images
EMAGE:671
Fig 1C Sporle et al, 1996 [PMID:8565855] Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: dMY, dorsal myotome; SB, somitic bud; vMY, ventral myotome.
Expression Pattern Description
Spatial Annotation:
EMAGE:671Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
671_wholemount_strong_3D_1.wlz
671_wholemount_moderate_3D_1.wlz
671_wholemount_weak_3D_1.wlz
671_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:671_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
trunk somite
detected detected
regionalExpression is in both dorsal and ventral myotome regions including the somitic bud
tail somite
detected detected
regionalExpression is in both dorsal and ventral myotome regions including the somitic bud
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1101192
Entity Detected:Myf5, myogenic factor 5 ( MGI:97252)
Sequence:sense strand is shown

>MGI:1101192
CCAACAGGCATCTGTCCTTGTTAATTACAGAGAGACAGTCCCAAACTCCGGGAGCTCCGCCTGGATTTGC
TGGCCTGCAGCAGCCAGGGACTGGCGTGTCTCCCTCTCTGCTGAATCCAGGTATTCCCACCTGCTTCTCT
GAAGGATGGACATGACGGACGGCTGCCAGTTCTCCCCTTCTGAGTACTTCTATGAAGGCTCCTGTATCCC
CTCACCAGAGGATGAGTTTGGGGACCAGTTTGAGCCAAGAGTAGCAGCCTTCGGAGCACACAAAGCTGAG
CTGCAGGGCTCAGACGATGAGGAGCACGTGCG
nt 159750 - nt 160061 of AC021642.14
Notes:The probe used in this study by Sporle et al, 1996 [PMID:8565855] is defined as "a 310 Nu riboprobe (Ott et al., 1991 [PMID:1652425] )". Ott describe the probe as a "BalI-ApaLI" genomic fragment "of 311 nucleotides from nucleotides 15 to 326 relative to the putative transcriptional start site of the mouse gene: 5'-CCAACAGGCATCTGTCCTTGTTAATTACAGAGAGACAGTCCCAAACTCCGGGAGCTCCGCCTGGATTTGCTGGCCTGCAGCAGCCAGGGACTCGGTGTCTCCCTCTCTGCTGAATCCAGGTATTCCCACCTGCTTCTCTGAAGGATGGACATGACGGACGGCTGCCAGTTCTCCCCTTCTGAGTACTTCTATGAAGGCTCCTGTATCCCCTCACCAGAGGATGAGTTTGGGGACCAGTTTGAGCCAAGAGTAGCAGCCTTCGGAGCACACAAAGCTGAGCTGCAGGGCTCAGACGATGAGGAGCACGTGCG-3' "
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Sporle et al, 1996 [PMID:8565855] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:8565855] Sporle R, Gunther T, Struwe M, Schughart K 1996 Severe defects in the formation of epaxial musculature in open brain (opb) mutant mouse embryos. Development (122):79-86
 [ PMID:1652425] Ott MO, Bober E, Lyons G, Arnold H, Buckingham M 1991 Early expression of the myogenic regulatory gene, myf-5, in precursor cells of skeletal muscle in the mouse embryo. Development (111):1097-107
Links:MGI:1336630 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI