EMAGE mouse gene expression spatial database

EMAGE:6727

Dnajc15 DnaJ (Hsp40) homolog, subfamily C, member 15 ( MGI:1913398)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002653. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002653_01	euxassay_002653_02	euxassay_002653_03	euxassay_002653_04

euxassay_002653_05	euxassay_002653_06	euxassay_002653_07	euxassay_002653_08	euxassay_002653_09

euxassay_002653_10	euxassay_002653_11	euxassay_002653_12	euxassay_002653_13	euxassay_002653_14

euxassay_002653_15	euxassay_002653_16	euxassay_002653_17	euxassay_002653_18	euxassay_002653_19

euxassay_002653_20	euxassay_002653_21	euxassay_002653_22	euxassay_002653_23	euxassay_002653_24

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Expression pattern clarity:
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Expression Pattern Description

Spatial Annotation:

Annotation colour key:

wholemount mapping

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Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal gland

strong

regional

strong expression: see section 06 07 08 17 18 moderate expression: see section 09 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2996
Entity Detected:	Dnajc15, DnaJ (Hsp40) homolog, subfamily C, member 15 ( MGI:1913398)
Sequence:	sense strand is shown >T2996 TGGCCTCGAGGCCAGATTCGGACGAGGATGATTTTAAACCATCCAGACAAAGGTGGATCTCCTTACTTAG CATCCAAAATAAATGAAGCAAAAGATTTGCTCGAAGCATCCAGCAAAGCTAACTGATGCTAAAGGACTGT ACATACCGAGGGAAAATGGAACAAACGCACAGCTGTAAAAGTCCTTCAGAAGAATGTGGCACGTGGTCGT GTTCCATACTGACCCAGTCTGTTTTCTGTCATTAAGTGTGCAGCAATAAAAGCCTGGCAGCCTTGCAAAA AAACAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:1533234 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1533234 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6729 same embryo
	EMAGE:6728 same embryo
	EMAGE:6730 same embryo
	EMAGE:6726 same embryo
	EurExpress:euxassay_002653 same experiment
	MGI:4824352 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source