EMAGE mouse gene expression spatial database

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EMAGE:6738

Rap2b RAP2B, member of RAS oncogene family ( MGI:1921262)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002574. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002574_01	euxassay_002574_02	euxassay_002574_03	euxassay_002574_04

euxassay_002574_05	euxassay_002574_06	euxassay_002574_07	euxassay_002574_08	euxassay_002574_09

euxassay_002574_10	euxassay_002574_11	euxassay_002574_12	euxassay_002574_13	euxassay_002574_14

euxassay_002574_15	euxassay_002574_16	euxassay_002574_17	euxassay_002574_18	euxassay_002574_19

euxassay_002574_20	euxassay_002574_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6738_wholemount_strong.wlz

6738_wholemount_moderate.wlz

6738_wholemount_weak.wlz

6738_wholemount_possible.wlz

6738_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6738_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

weak

regional

weak expression: see section 07 08 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3331
Entity Detected:	Rap2b, RAP2B, member of RAS oncogene family ( MGI:1921262)
Sequence:	sense strand is shown >T3331 TGGCCTCGAGGCCAGATTCGGCACGAGGGTGGACTCGTCGCCGTCGGTGCTGGAGATCTTGGACACCGCG GGCACGGAGCAGTTCGCCTCAATGCGGGACCTGTACATCAAGAATGGCCAGGGCTTCATTCTCGTCTACA GCCTGGTCAACCAGCAGAGCTTCCAGGACATCAAGCCCATGCGGGACCAGATCATCCGCGTGAAGCGGTA CGAGCGCGTACCCATGATCCTGGTAGGCAACAAGGTGGACTTGGAGGGTGAACGTGAGGTCTCCTATGGC GAGGGTAAGGCCCTGGCCGAGGAGTGGAGCTGCCCCTTCATGGAGACATCGGCCAAAAACAAAGCCTCAG TGGATGAGCTATTCGCAGAGATCGTGAGGCAGATGAACTACGCGGCACAGCCCAACGGCGACGAGGGCTG CTGCTCGGCCTGCGTGATCCTGTGAGGCGCCGTCTGCCGCCGGGCGCTGGCCACGCTCTCTGCACAAAGC CAAACGCACCCGATTCTCTTAATGTGATTGTCTTCTTGCTTTGAGATTGGAGACGACTTTG
Notes:	The probe template was PCR amplified from IMAGE:314317 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:314317 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6740 same embryo
	EMAGE:6737 same embryo
	EMAGE:6739 same embryo
	EurExpress:euxassay_002574 same experiment
	MGI:4827610 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source