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EMAGE:6786

Scg5 secretogranin V ( MGI:98289)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:6786 EMAGE:6786 EMAGE:6786 EMAGE:6786 EMAGE:6786
"Pseudo-wholemount" of euxassay_007348. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_007348_01 euxassay_007348_02 euxassay_007348_03 euxassay_007348_04
EMAGE:6786 EMAGE:6786 EMAGE:6786 EMAGE:6786 EMAGE:6786
euxassay_007348_05 euxassay_007348_06 euxassay_007348_07 euxassay_007348_08 euxassay_007348_09
EMAGE:6786 EMAGE:6786 EMAGE:6786 EMAGE:6786 EMAGE:6786
euxassay_007348_10 euxassay_007348_11 euxassay_007348_12 euxassay_007348_13 euxassay_007348_14
EMAGE:6786 EMAGE:6786 EMAGE:6786 EMAGE:6786 EMAGE:6786
euxassay_007348_15 euxassay_007348_16 euxassay_007348_17 euxassay_007348_18 euxassay_007348_19
EMAGE:6786 EMAGE:6786 EMAGE:6786 EMAGE:6786 EMAGE:6786
euxassay_007348_20 euxassay_007348_21 euxassay_007348_22 euxassay_007348_23 euxassay_007348_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:6786Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
6786_wholemount_strong.wlz
6786_wholemount_moderate.wlz
6786_wholemount_weak.wlz
6786_wholemount_possible.wlz
6786_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:6786_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
brain
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
pituitary gland
strong strong
regionalstrong expression: see section 10 11 12 13 14 15 16
facial vii ganglion
strong strong
regionalstrong expression: see section 05 06 07 18 19 20 21
glossopharyngeal ix ganglion
strong strong
regionalstrong expression: see section 07 08 19 20
trigeminal v ganglion
strong strong
regionalstrong expression: see section 04 05 06 07 08 09 16 17 18 19 20 21 22
vagus x ganglion
strong strong
regionalstrong expression: see section 08 19
vestibulocochlear viii ganglion
strong strong
regionalstrong expression: see section 07 08 09 18 19 20
trigeminal v nerve
strong strong
regionalstrong expression: see section 08 09 16
spinal cord
strong strong
regionalstrong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18
cervico-thoracic ganglion
strong strong
regionalstrong expression: see section 09 10 13 14 15 16 17
cervical ganglion
strong strong
regionalstrong expression: see section 09 10 16 17 18
thoracic ganglion
strong strong
regionalstrong expression: see section 09 10 11 12 13
dorsal root ganglion
strong strong
regionalstrong expression: see section 08 09 10 11 12 13 14 15 16 17 18 19
neural retina
strong strong
regionalstrong expression: see section 01 02 03 21 22 23 24
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 09 10 13 14 15 weak expression: see section 07 08 16 17
esophagus
strong strong
spottedstrong expression: see section 13 14 moderate expression: see section 12
tongue
strong strong
spottedstrong expression: see section 11 13 14
stomach
strong strong
spottedstrong expression: see section 01 02 03 04 05 06 07 08 09 10
midgut
strong strong
spottedstrong expression: see section 10 11 12 13 14 15 16 17 18 19 20 21 22 moderate expression: see section 23
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T3015
Entity Detected:Scg5, secretogranin V ( MGI:98289)
Sequence:sense strand is shown

>T3015
TGGCTCGAGCAGATTCGGACGAGGCCTGCGGCCACACGGTTAAAAATGGCCTCAAGGCTGGTCTCTGCCA
TGCTATCTGGCCTTTTGTTTTGGTTGATGTTTGAGTGGAATCCAGCATTCGCTTATAGTCCACGGACCCC
TGACCGGGTCTCAGAGACAGACATCCAGAGGCTGCTTCATGGGGTTATGGAGCAGCTGGGCATTGCCAGG
CCACGCGTGGAGTACCCGGCTCACCAGGCCATGAATCTTGTTGGGCCGCAGAGCATCGAAGGGGGAGCTC
ACGAGGGTCTTCAGCATCTGGGTCCTTTTGGCAACATCCCCAACATAGTGGCAGAGTTGACTGGTGACAA
CATTCCTAAGGACTTCAGTGAGGATCAAGGCTACCCAGACCCTCCAAATCCCTGTCCTCTTGGGAAAACT
GCTGATGATGGATGTCTAGAAAACGCCCCCGACACTGCAGAGTTCAGCCGAGAATTCCAGTTAGACCAGC
ACCTTTTTTATCCAGAACATGACTACCCAGGTTTGGGCAAGTGGAACAAGA
Notes:The probe template was PCR amplified from IMAGE:1533489 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1533489 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:6783 same embryo
 EMAGE:6782 same embryo
 EMAGE:6785 same embryo
 EMAGE:6787 same embryo
 EMAGE:6784 same embryo
 EurExpress:euxassay_007348 same experiment
 MGI:4827896 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS