EMAGE mouse gene expression spatial database

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EMAGE:6797

Stat4 signal transducer and activator of transcription 4 ( MGI:103062)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002286. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002286_01	euxassay_002286_02	euxassay_002286_03	euxassay_002286_04

euxassay_002286_05	euxassay_002286_06	euxassay_002286_07	euxassay_002286_08	euxassay_002286_09

euxassay_002286_10	euxassay_002286_11	euxassay_002286_12	euxassay_002286_13	euxassay_002286_14

euxassay_002286_15	euxassay_002286_16	euxassay_002286_17	euxassay_002286_18	euxassay_002286_19

euxassay_002286_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6797_wholemount_strong.wlz

6797_wholemount_moderate.wlz

6797_wholemount_weak.wlz

6797_wholemount_possible.wlz

6797_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6797_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

weak

regional

weak expression: see section 08 09 10 11 12 13

axial skeleton

moderate

regional

moderate expression: see section 10 11 12 13

nucleus pulposus

moderate

regional

moderate expression: see section 10 11 12 13

basioccipital bone

weak

regional

weak expression: see section 01 02 03 17 18 19

basisphenoid bone

weak

regional

weak expression: see section 09 10 11

temporal bone

weak

regional

weak expression: see section 03 19

orbito-sphenoid

weak

regional

weak expression: see section 01 02 03 04 05 09 10 11 16 17 18 19

viscerocranium

weak

regional

Expression in the turbinate bone.

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1822
Entity Detected:	Stat4, signal transducer and activator of transcription 4 ( MGI:103062)
Sequence:	sense strand is shown >T1822 TGGCTCGAGNCAGATTCGGACGAGGGGTCACCTCACCTGGGCCAAGTTCTGCAAGGAACATTTGCCTGGC AAAACATTTACCTTCTGGACTTGGCTTGAAGCAATATTGGACCTAATTAAAAAACATATTCTTCCCCTCT GGATTGATGGGTACATCATGGGATTTGTTAGTAAAGAGAAGGAACGGCTTCTGCTCAAAGATAAAATGCC TGGGACATTTTTGTTAAGATTCAGTGAGAGCCATCTTGGAGGGATAACCTTCACCTGGGTGGACCAATCT GAAAATGGAGAAGTGAGATTCCACTCTGTAGAACCCTACAACAAAGGGAGACTGTCGGCTCTGCCGTTCG CTGACATCCTGCGAGACTACAAGGTTATCATGGCTGAAAACATCCCTGAAAACCCTCTGAAGTACCTCTA CCCTGACATTCCCAAAGACAAAGCCTTTGGCAAACACTACAGCTCCCAGCCGTGCGAAGTCTCAAGACCA ACCGAACGGGGAGACAAGGGTTACGTCCCCTCTGTTTTTATCCCCATTTCAA
Notes:	The probe template was PCR amplified from IMAGE:577343 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:577343 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6796 same embryo
	EMAGE:6800 same embryo
	EMAGE:6799 same embryo
	EMAGE:6798 same embryo
	EMAGE:6795 same embryo
	EurExpress:euxassay_002286 same experiment
	MGI:4828487 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source