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EMAGE:685

Nodal nodal ( MGI:97359)
TS10 (6.75 dpc)
in situ hybridisation

Data Images
EMAGE:685
Figs 7e,f Perea-Gomez et al, 2001 [PMID:11171400] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Arrow in F points to anterior epiblast.
Expression Pattern Description
Spatial Annotation:
EMAGE:685EMAGE:685Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D context movie

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
685_voxel_strong_3D_1.wlz
685_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:685_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
embryo ectoderm
detected detected
regionalExpression is detected in the posterior epiblast
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1334976
Entity Detected:Nodal, nodal ( MGI:97359)
Sequence:sense strand is shown

>MGI:1334976
TTTTTGAGCCAAGAAGAGGATCTGGTATGGGCGGACGTCCGGTTGCAGCTGCCGGGCCCCATGGACATAC
CCACTGAGGGCCCACTCACCATTGACATTTTCCACCAGGCCAAGGGGGATCCAGAGCGGGACCCCGCTGA
CTGCCTGGAGCGCATTTGGATGGAGACGTTCACCGTCATTCCTTCTCAGGTCACGTTTGCCTCAGGCAGC
ACAGTCCTGGAGGTGACCAAGCCACTCTCCAAGTGGCTAAAGGACCCCAGGGCACTGGAAAAGCAGGTGT
CCAGTCGAGCAGAAAAGTGTTGGCATCAGCCCTACACCCCACCTGTACCTGTCGCCAGCACCAATGTGCT
CATGCTCTACTCCAACCGGCCTCAGGAGCAGAGGCAGCTAGGGGGCGCCACTTTGCTTTGGGAAGCTGAG
AGCTCCTGGCGGGCCCAGGAGGGACAGCTGTCTGTAGAGAGGGGCGGATGGGGCAGAAGGCAACGCCGAC
ATCATTTGCCAGACAGAAGCCAACTGTGTAGGAGGGTCAAGTTCCAGGTGGACTTCAACCTGATTGGCTG
GGGCTCCTGGATCATCTACCCCAAGCAGTACAATGCCTATCGCTGTGAGGGCGAGTGTCCTAACCCTGTG
GGGGAGGAGTTTCATCCTAC
nt 571 - nt 1220 of X70514.1
Notes:The probe used in this study by Perea-Gomez et al, 2001 [PMID:11171400] is described as that used by Zhou et al., 1993 [PMID:8429908] . Zhou et al describe the probe as a "630-bp nodal cDNA fragment (nucleotides 571-1220) cloned in pBluescript SK". See Fig 2a therein for sequence.
Chemistry:RNA
Strand:antisense
Label:S35
Specimen
Organism:mouse
Age:6.75 dpc
Theiler Stage:TS10
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Embedding:cryosection
Staining procedure:autoradiography
General Information
Authors:Perea-Gomez et al, 2001 [PMID:11171400] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:11171400] Perea-Gomez A, Lawson KA, Rhinn M, Zakin L, Brulet P, Mazan S, Ang SL 2001 Otx2 is required for visceral endoderm movement and for the restriction of posterior signals in the epiblast of the mouse embryo. Development (128):753-65
 [ doi:10.1038/361543a0] [ PMID:8429908] Zhou X, Sasaki H, Lowe L, Hogan BL, Kuehn MR 1993 Nodal is a novel TGF-beta-like gene expressed in the mouse node during gastrulation. Nature (361):543-7
Links:MGI:1930294 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI