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EMAGE:6854

Zfp318 zinc finger protein 318 ( MGI:1889348)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:6854 EMAGE:6854 EMAGE:6854 EMAGE:6854 EMAGE:6854
"Pseudo-wholemount" of euxassay_002356. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_002356_01 euxassay_002356_02 euxassay_002356_03 euxassay_002356_04
EMAGE:6854 EMAGE:6854 EMAGE:6854 EMAGE:6854 EMAGE:6854
euxassay_002356_05 euxassay_002356_06 euxassay_002356_07 euxassay_002356_08 euxassay_002356_09
EMAGE:6854 EMAGE:6854 EMAGE:6854 EMAGE:6854 EMAGE:6854
euxassay_002356_10 euxassay_002356_11 euxassay_002356_12 euxassay_002356_13 euxassay_002356_14
EMAGE:6854 EMAGE:6854 EMAGE:6854 EMAGE:6854 EMAGE:6854
euxassay_002356_15 euxassay_002356_16 euxassay_002356_17 euxassay_002356_18 euxassay_002356_19
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euxassay_002356_20 euxassay_002356_21 euxassay_002356_22 euxassay_002356_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:6854Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
6854_wholemount_strong.wlz
6854_wholemount_moderate.wlz
6854_wholemount_weak.wlz
6854_wholemount_possible.wlz
6854_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:6854_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T1942
Entity Detected:Zfp318, zinc finger protein 318 ( MGI:1889348)
Sequence:sense strand is shown

>T1942
TGGCCTCGAGCCAGATTCGGCACGAGGGTAGTGGTGGAAAGGGGGCCCATGAGACTAAGCTGAGTAGTAG
TACATTAGCCAATGGGGAAAGCAGTAGCCTTCCTAGAACAGAAAGTTCAGATTTCTCCTCCACTTGTACT
TTGAACAGTAGTATGTCCTCAGAGGACCTGCCTCAATGTAGTGCTTTGGTCACTGCTACTGAAATCAGTA
ATCTTGAAAATCCTATTTCAAAAGGTATGGAGTCCACAGGGAAATGGTCTGTTGTAGATCAGATAGATCC
CAAAAGCAGAGACAGCACCTATAGCTTCCTACAGCCTCTGACAAGATTATACCAAAATAAGCCTTATGAA
ATAGTTTCCCCAAAGACAGACACTTTGGTCATGTGGACTTCTGGTTCTTCCCAGAATGACACTCATAAAG
ATAGACCTCCAGAGGGGAAAATCAGATTTGACCTGGGAGAGCCAGGGCCACCTGGTACAGATTCAACTTC
TCATCTATCAGATACACACTGTCAAACCAACGGACCTCAGAAGTTGATAGAAATTAACCTCATAGATAAC
CAAAA
Notes:The probe template was PCR amplified from IMAGE:636449 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:636449 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:6855 same embryo
 EMAGE:6856 same embryo
 EMAGE:6853 same embryo
 EMAGE:6857 same embryo
 EMAGE:6852 same embryo
 EurExpress:euxassay_002356 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS