EMAGE mouse gene expression spatial database

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EMAGE:6893

Prdm8 PR domain containing 8 ( MGI:1924880)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003278. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003278_01	euxassay_003278_02	euxassay_003278_03	euxassay_003278_04

euxassay_003278_05	euxassay_003278_06	euxassay_003278_07	euxassay_003278_08	euxassay_003278_09

euxassay_003278_10	euxassay_003278_11	euxassay_003278_12	euxassay_003278_13	euxassay_003278_14

euxassay_003278_15	euxassay_003278_16	euxassay_003278_17	euxassay_003278_18	euxassay_003278_19

euxassay_003278_20	euxassay_003278_21	euxassay_003278_22	euxassay_003278_23	euxassay_003278_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6893_wholemount_strong.wlz

6893_wholemount_moderate.wlz

6893_wholemount_weak.wlz

6893_wholemount_possible.wlz

6893_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6893_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon

weak

regional

weak expression: see section 08 09 10 11 12 13 14 15 16 17 18

telencephalon

moderate

regional

moderate expression: see section 01 02 03 weak expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

hindbrain

weak

regional

weak expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

midbrain

weak

regional

weak expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19

trigeminal v ganglion

weak

regional

weak expression: see section 03 04 05 06 07 08 18 19 20 21 22

spinal cord

weak

regional

weak expression: see section 09 10 11 12 13 14 15 16

dorsal root ganglion

moderate

regional

moderate expression: see section 17 weak expression: see section 07 08 09 10 11 12 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3340
Entity Detected:	Prdm8, PR domain containing 8 ( MGI:1924880)
Sequence:	sense strand is shown >T3340 TGGCCTCGAGGCCAGATTCGGACGAGGTGNCCTCCGACCTGGTGTACCATATGCGGTCTCACCACAAAAA GGAGTATGCCATGGAGCCCCTGGTGAAGCGGCGGCGGGAGGAGAAACTCAAGTGCCCCATTTGCAACGAG TCCTTCAGGGAGCGTCACCACCTGTCCCGGCACATGACCTCGCATAATTAATTGTTGCGTAAAAGACTCC GGCTTTCCACGCGTGTGCACGCACAGTCAAGCCACCTGCAGGAGCAGACACGCGAGGATGACATCCACCA CTACCTTATGCCCCCAAACACTGCAACCAAACACGTGCACACACAGGTCCCTCCCCTCGCCAGGAGCCTC ATCATCCAAATGTTTAATTTCACACACACACACACACACACACACACACACACACACACACACGCACGCA CGAACGCACACACGGACGCACGCACACGATGCACAGGATGGTGGATTTTGGATTGGGTGGGTTGTTTGAG CGGGGTTTTCTTTTGAATGCACGAATTTTCACTTTCCCAAAAACAAAAGTACATTTTTAAAAAAATGTCA TAT
Notes:	The probe template was PCR amplified from IMAGE:315997 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:315997 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6892 same embryo
	EurExpress:euxassay_003278 same experiment
	MGI:4827400 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source