EMAGE mouse gene expression spatial database

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EMAGE:6926

Akap11 A kinase (PRKA) anchor protein 11 ( MGI:2684060)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007645. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007645_01	euxassay_007645_02	euxassay_007645_03	euxassay_007645_04

euxassay_007645_05	euxassay_007645_06	euxassay_007645_07	euxassay_007645_08	euxassay_007645_09

euxassay_007645_10	euxassay_007645_11	euxassay_007645_12	euxassay_007645_13	euxassay_007645_14

euxassay_007645_15	euxassay_007645_16	euxassay_007645_17	euxassay_007645_18	euxassay_007645_19

euxassay_007645_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6926_wholemount_strong.wlz

6926_wholemount_moderate.wlz

6926_wholemount_weak.wlz

6926_wholemount_possible.wlz

6926_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6926_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vertebral axis musculature

weak

regional

weak expression: see section 01 02 03 04 05 06 14 15 16 17 18 19 20

forebrain

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

hindbrain

weak

regional

weak expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

midbrain

weak

regional

weak expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17

facial vii ganglion

moderate

regional

moderate expression: see section 02 03 17 18 19

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 04 05 15 16

trigeminal v ganglion

moderate

regional

moderate expression: see section 01 02 03 17 18 19 20 weak expression: see section 04 05 06 07 15 16

vagus x ganglion

weak

regional

weak expression: see section 06 15

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 04 05 06 15 16

trigeminal v nerve

weak

regional

weak expression: see section 07 15

spinal cord

weak

regional

weak expression: see section 04 05 06 07 08 09 10 11 12

dorsal root ganglion

weak

regional

weak expression: see section 03 04 05 06 08 09 10 11 12 13 14 15

neural retina

weak

regional

weak expression: see section 01 02

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35918
Entity Detected:	Akap11, A kinase (PRKA) anchor protein 11 ( MGI:2684060)
Sequence:	sense strand is shown >T35918 CTCTTTTCAAAGCAGAGTCCGTGTGCTAGTGCTAAGCCAAGTTCACGTTCAAAACTTAGTAGCATTCGTC AGAAATCAAGAATTTTTCATCTTGATGTACCTCAGATTCATGTTAATCTTGATAAAAGGGCAGTGCTTGC TGAGAAGATAGTTGCTGAAGCTATTGAGAAAGCAGAGAGAGAGTTGAGCAACACGAGTTTGGCAGCTGAT AGTGGAATCGGACAAGATGGCATTAGCTTTGCTGAGAGCCTTACTACAGAAATCATGACAACAGCTGTGA CAAATGCTGGGCATGCTGTTAGCAGTTCAAGAGAAATAGAAGATTTTCAGTCAACTGAGTCACTTGGCAG CCAGCAGATGAATCTCAGTGTTGGTGAAGACAGCACAGGTAGCTGGTCCAACTTAAGTTTTGAGGATGAC CATCAAGATGAAAGCAGCAGTTTCCATCATCTCAGTGAAAGCAGTAATGGTAACAGCAGTAGCTGGAGCA GTCTTGGTTTAGAAGGAGATTTGTATGAGGACAATTTATCCTTTCCAACATCAGACAGTGATGGATCGGA TGATGGAGATGACGAGCAGGAGGATGGTGTGGAAGATTTGCAGCAAAATGGAAAGACTCTGCTAATTATG AACATTGACATGGAGCCTGGAGCTGTAGACCCGCAATTAAGGATTATTCTTCAGTGGCTCATTGCCTCTG AGGCTGAAGTTGCAGAACTTTA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 84831. Forward Primer - name:084831_F_cDNA_Akap11, sequence:CTCTTTTCAAAGCAGAGTCCGT; Reverse Primer - name:084831_N_SP6_cDNA_Akap11, sequence:TAAAGTTCTGCAACTTCAGCC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6924 same embryo
	EMAGE:6927 same embryo
	EMAGE:6923 same embryo
	EMAGE:6928 same embryo
	EMAGE:6925 same embryo
	EurExpress:euxassay_007645 same experiment
	MGI:4823060 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source