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EMAGE:6932

Adra1b adrenergic receptor, alpha 1b ( MGI:104774)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:6932 EMAGE:6932 EMAGE:6932 EMAGE:6932 EMAGE:6932
"Pseudo-wholemount" of euxassay_002511. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_002511_01 euxassay_002511_02 euxassay_002511_03 euxassay_002511_04
EMAGE:6932 EMAGE:6932 EMAGE:6932 EMAGE:6932 EMAGE:6932
euxassay_002511_05 euxassay_002511_06 euxassay_002511_07 euxassay_002511_08 euxassay_002511_09
EMAGE:6932 EMAGE:6932 EMAGE:6932 EMAGE:6932 EMAGE:6932
euxassay_002511_10 euxassay_002511_11 euxassay_002511_12 euxassay_002511_13 euxassay_002511_14
EMAGE:6932 EMAGE:6932 EMAGE:6932 EMAGE:6932 EMAGE:6932
euxassay_002511_15 euxassay_002511_16 euxassay_002511_17 euxassay_002511_18 euxassay_002511_19
EMAGE:6932
euxassay_002511_20

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:6932Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
6932_wholemount_strong.wlz
6932_wholemount_moderate.wlz
6932_wholemount_weak.wlz
6932_wholemount_possible.wlz
6932_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:6932_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T2056
Entity Detected:Adra1b, adrenergic receptor, alpha 1b ( MGI:104774)
Sequence:sense strand is shown

>T2056
TGGCCTCGAGCCAGATTCGGACGAGGGAGAATGATCTTTAATATTTGACTTGTTTTCCGACCCTATCAAA
GGGCCAGCATTGACTGGAGAGCAGGGAGGGGTTTGAGAGGTACAAAGTCAAGAAACAAACAACACACCTA
GACATCACCAGGGGTCACACTCACTCTGCCCCAGCACATGCTTGCGGGCAGGCCTCACACACGCACCCAC
GCAGCCCACACGTACACCAAACAGACACACGCACGTGTACCCACAGATAAATACATATAGAGGAACTCAT
TTTATGTATATACAGAGAGAGAAGAGAGAGAGACTTAAGTCCCGGCCGGCGTAGGGGGGGCCCCCAGGCT
CTCAGCATGCTCAGCCCAGCTCTTCGCTGTACCTGGCACCCATGCCTCTGCCAGAAAAGCAGCTGCCCCT
CTCCCCACCCCCTCCCCTTTGTCCCCTTGAGACCTTGTGTCTACTCAGGACTGACACACTCCCCCCACGC
GCCCTCCCACCTCCCATGATGGTGTTCTTCCCCCAACCCCCGATGTGTGTGTGTTCGGGGGCGGGTGGAT
GAAGA
Notes:The probe template was PCR amplified from IMAGE:809273 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:809273 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:6930 same embryo
 EMAGE:6933 same embryo
 EMAGE:6934 same embryo
 EMAGE:6935 same embryo
 EMAGE:6929 same embryo
 EMAGE:6931 same embryo
 EurExpress:euxassay_002511 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS