EMAGE mouse gene expression spatial database

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EMAGE:6933

Bcl11b B-cell leukemia/lymphoma 11B ( MGI:1929913)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002403. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002403_01	euxassay_002403_02	euxassay_002403_03	euxassay_002403_04

euxassay_002403_05	euxassay_002403_06	euxassay_002403_07	euxassay_002403_08	euxassay_002403_09

euxassay_002403_10	euxassay_002403_11	euxassay_002403_12	euxassay_002403_13	euxassay_002403_14

euxassay_002403_15	euxassay_002403_16	euxassay_002403_17	euxassay_002403_18	euxassay_002403_19

euxassay_002403_20	euxassay_002403_21	euxassay_002403_22	euxassay_002403_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6933_wholemount_strong.wlz

6933_wholemount_moderate.wlz

6933_wholemount_weak.wlz

6933_wholemount_possible.wlz

6933_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6933_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3883
Entity Detected:	Bcl11b, B-cell leukemia/lymphoma 11B ( MGI:1929913)
Sequence:	sense strand is shown >T3883 GCGGCTCAGACCCCCTCCCCGGCCCGCATCTGTGCAGCTTTCCGGGCGATGCCAGAATAGATGCCGGGGC AATGTCCCGCCGCAAACAGGGCAACCCGCAGCACTTGTCCCAGAGGGAACTCATCACGCCAGAGGCTGAC CATGTGGAGGCTACCATCCTCGAGGAAGACGAGGGTCTGGAGATAGAGGAGCCTAGCAGCCTGGGGCTGA TGGTGGGAGGCCCCGACCCTGATCTACTCACCTGTGGCCAGTGTCAGATGAACTTCCCGCTGGGGGACAT CCTGGTTTTTATAGAGCACAAGAAGAAACAGTGTGGAGGCCTGGGCCCCTGCTACGACAAGGTCCTGGAC AAGAGCAGTCCACCTCCCTCCTCTCGCTCTGAGCTCAGGAGAGTATCTGAGCCAGTGGAGATCGGGATCC AGGTCACCCCTGATGAAGATGACCACCTACTGTCACCCACGAAAGGCATCTGTCCCAAGCAGGAGAACAT TGCAGGTAAAGATGAGCCTTCCAGCTACATTTGCACAACATGCAAGCAGCCCTTCAACAGCGCCTGGTTC CTGCTGCAGCACGCACAGAACACGCATGGCTTCCGAATCTACCTGGAGCCTGGGCCGGCCAGCACCTCGC TCACGCCCAGGCTCACCATCCCGCCACCGCTCGGGCCGGAGACCGTGGCGCAGTCCCCACTCATGAATTT CCTGGGGGACAGCAATCCTTTCAACCTGCTGCGCATGACGGGCCCCATCCTGCGGGACCACCCTGGCTTC GGTGAGGGCCGCTTGCCAGGTACGCCACCGCTCTTCAGCCCACCGCCACGCCATCACTTGGACCCACACC GCCTCAGTGCAGAGGAGATGGGGCTCGTGGCCCAGCACCCCAGTGCCTTCGACCGAGTCATGCGCCTGAA CCCCATGGCCATAGACTCTCCTGCCATGGACTTCTCCCGGCGGCTGCGAGAACTGGCCGGCAACAGCTCC ACGCCGCCGCCCGTGTCCCCAGGCCGTGGCAACCCTATGCACCGGCTGCTGAACCCTTTCCAGCCCAGTC CCAAGTCCCCGTTCCTCAGCACGCCACCGCTGCCACCCATGCCTGCGGGCACACCGCCACCGCAGCCGCC TGCCAAGAGCAAGTCCTGTGAGTTCTGCGGCAAGACCTTCAAGTTCCAGAGCAATCTCATCGTGCACCGG CGCAGCCACACGGGCGAGAAGCCCTACAAGTGCCAGCTGTGCGACCATGCGTGCTCGCAGGCGAGCAAGC TCAAGCGCCACATGAAGACGCACATGCACAAGGCGGGCTCTCTGGCTGGCCGCTCAGACGACGGGCTCTC AGCTGCCAGCTCCCCTGAGCCGGGCACCAGCGAGCTGCCAGGTGACCTGAAAGCGGCCGATGGCGACTTC CGCCACCATGAGAGCGACCCATCTCTGGGCCCCGAGCCTGAGGACGACGAGGACGAGGAGGAGGAAGAAG AGGAGCTGCTGCTGGAGAACGAGAGCCGGCCTGAGTCGAGCTTCAGCATGGACTCGGAGCTGGGCCGTGG CCGCGAGAACGGAGGTGGTGTGCCACCGGGGGTGGCGGGCGCAGGGGCTGCAGCTGCGGCTCTGGCGGAT GAGAAGGCTCTGGCCCTGGGCAAGGTGATGGAGGACGCAGGGCTGGGCGCACTGCCGCAGTATGGGGAGA AGCGGGGCGCCTTCCTGAAGCGTGCAGGCGACACGGGTGATGCCGGAGCTGTTGGCTGTGGGGACGCGGG TGCACCGGGTGCAGTGAACGGGCGCGGCGGGGCCTTCGCGCCAGGCGCAGAGCCCTTTCCAGCTCTCTTC CCACGCAAGCCAGCACCGCTGCCCAGCCCTGGGCTCGGTGGTCCCGCGCTGCACGCGGCCAAGCGCATCA AGGTGGAGAAAGACCTGGAGCTGCCACCTGCCGCCCTCATCCCATCTGAGAACGTGTACTCGCAGTGGCT CGTGGGCTACGCAGCATCGCGCCACTTCATGAAGGACCCATTCCTGGGCTTCACGGATGCGCGCCAGTCG CCTTTCGCCACATCGTCGGAACATTCCTCTGAGAACGGCAGCCTGCGCTTCTCAACGCCACCCGGGGACC TGCTGGACGGCGGGCTGTCCGGGCGCAGTGGCACGGCGAGCGGGGGCAGCACACCTCACCTGGGTGGTCC GGGTCCTGGGAGGCCGAGCTCCAAGGAGGGCCGCCGCAGCGACACATGTGAGTACTGCGGCAAGGTCTTC AAGAACTGTAGCAACCTGACGGTGCACCGGAGGAGCCACACCGGCGAGCGGCCTTACAAGTGCGAGCTGT GCAACTACGCGTGTGCGCAGAGCAGCAAGCTCACGCGCCACATGAAGACGCACGGGCAGATCGGCAAGGA GGTGTACCGCTGCGACATCTGCCAGATGCCCTTCAGCGTCTACAGCACCCTGGAGAAACACATGAAAAAG TGGCACGGTGAACACTTGCTGACTAATGATGTCAAAATCGAGCAGGCTGAGAGGAGCTAAGCGCATACGT GGGGGACACTGCGTGCGTGCGTCTGTACAGCGTGACCATCGCCAACCTTCGCCAACGGGACCGGTGACCG GACTGGCCTCTGCATCCCCGGGGCCCAGGGAGGCGGCAGTCCAACCTAACCTGTGTCTGCGAAGTCCTAT GGAAACCTGAGGGTTGATTAAGGCAGTAAAAAATTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4457123 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4457123 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6930 same embryo
	EMAGE:6934 same embryo
	EMAGE:6935 same embryo
	EMAGE:6929 same embryo
	EMAGE:6932 same embryo
	EMAGE:6931 same embryo
	EurExpress:euxassay_002403 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source