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EMAGE:697

Fgf5 fibroblast growth factor 5 ( MGI:95519)
TS08 (6.5 dpc)
in situ hybridisation

Data Images
EMAGE:697 EMAGE:697 EMAGE:697
Fig 1F Hebert et al., 1991 [PMID:1794311] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright. Fig 1D Hebert et al., 1991 [PMID:1794311] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright. Fig 1E Hebert et al., 1991 [PMID:1794311] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Image annotations: ec, embryonic ectoderm; ex, extraembryonic ectoderm; pe, parietal endoderm; ve, visceral endoderm. Cartoon representaion of the stage is shown in Fig1D on the right.
Expression Pattern Description
Spatial Annotation:
EMAGE:697EMAGE:697Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D context movie

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
697_voxel_strong_3D_1.wlz
697_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:697_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
epiblast
strong strong
Fgf5 mRNA was readily detectable at uniformly high levels throughout the embryonic ectoderm at this stage
extraembryonic component
not detected not detected
regionalFgf5 mRNA was undetectable in the proximal extraembryonic tissue
primitive endoderm
possible possible
Authors state that it was difficult to determine whether Fgf5 mRNA was expressed in visceral endoderm cells
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:17266
Entity Detected:Fgf5, fibroblast growth factor 5 ( MGI:95519)
Sequence:sense strand is shown

>MGI:17266
ATGAGCCTGTCCTTGCTCTTCCTCATCTTCTGCAGCCACCTGATCCACAGCGCTTGGGCTCACGGGGAGA
AGCGTCTCACTCCCGAAGGGCAACCCGCGCCTCCTAGGAACCCGGGAGACTCCAGCGGCAGCCGGGGCAG
AAGTAGCGCGACGTTTTCTTCGTCTTCTGCCTCCTCACCAGTCGCAGCTTCTCCGGGCAGCCAAGGAAGC
GGCTCGGAACATAGCAGTTTCCAGTGGAGCCCTTCGGGGCGCCGGACCGGCAGCCTGTACTGCAGAGTGG
GCATCGGTTTCCATCTGCAGATCTACCCGGATGGCAAAGTCAATGGCTCCCACGAAGCCAGTGTGTTAAG
TATTTTGGAAATATTTGCTGTGTCTCAGGGGATTGTAGGAATACGAGGAGTTTTCAGCAACAAATTTTTA
GCGATGTCAAAAAAAGGAAAACTCCATGCAAGTGCCAAATTTACGGATGACTGTAAGTTCAGGGAGAGAT
TCCAAGAAAACAGCTATAATACCTATGCGTCCGCGATCCACAGAACTGAAAAGACAGGCCGAGAGTGGTA
CGTGGCCCTGAACAAGAGAGGGAAAGCCAAGAGAGGCTGCAGCCCACGAGTCAAACCCCAACACGTCTCC
ACCCACTTCCTACCCAGGTTCAAGCAGTCCGAGCAACCGGAACTTTCCTTCACCGTCACTGTTCCAGAAA
AGAAAAAGCCACCGGTGAAACCAAAGGTGCCCCTGTCGCAGCCTCGCAGAAGTCCCAGCCCAGTGAAGTA
CAGACTGAAGTTTCGCTTTGGATGA
nt 1 - nt 795 of M30643.1
Notes:The probe used in this study by Hebert et al., 1991 [PMID:1794311] is described as the "full-length Fgf-5 coding sequence (Hebert et al, 1990 [PMID:2318343] )". The GenBank accession ID for Fgf5 submitted by Hebert et al, 1990 is M30643.
Chemistry:RNA
Strand:antisense
Label:S35
Specimen
Organism:mouse
Strain:ICR
Age:6.5 dpc
Theiler Stage:TS08
Mutations:none (wild-type)
Preparation:section
Procedures
Staining procedure:autoradiography
General Information
Authors:Hebert et al., 1991 [PMID:1794311] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:1794311] Hebert JM, Boyle M, Martin GR 1991 mRNA localization studies suggest that murine FGF-5 plays a role in gastrulation. Development (112):407-15
 [ doi:10.1016/0012-1606(90)90211-Z] [ PMID:2318343] H�bert JM, Basilico C, Goldfarb M, Haub O, Martin GR 1990 Isolation of cDNAs encoding four mouse FGF family members and characterization of their expression patterns during embryogenesis. Dev Biol (138):454-63
Links:MGI:1930524 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI