EMAGE mouse gene expression spatial database

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EMAGE:6983

Trappc6a trafficking protein particle complex 6A ( MGI:1914341)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007527. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007527_01	euxassay_007527_02	euxassay_007527_03	euxassay_007527_04

euxassay_007527_05	euxassay_007527_06	euxassay_007527_07	euxassay_007527_08	euxassay_007527_09

euxassay_007527_10	euxassay_007527_11	euxassay_007527_12	euxassay_007527_13	euxassay_007527_14

euxassay_007527_15	euxassay_007527_16	euxassay_007527_17	euxassay_007527_18	euxassay_007527_19

euxassay_007527_20	euxassay_007527_21	euxassay_007527_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6983_wholemount_strong.wlz

6983_wholemount_moderate.wlz

6983_wholemount_weak.wlz

6983_wholemount_possible.wlz

6983_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6983_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5814
Entity Detected:	Trappc6a, trafficking protein particle complex 6A ( MGI:1914341)
Sequence:	sense strand is shown >T5814 CCACGCGTCCGCCCACGCGTCCGCGGACGCGTGGGCGTGAGGGTCGCGAGGCGGCGTATGGCGGACGCGG TGCTATTCGAGTTTCTGCACACCGAGATGGTGGCGGAGCTGTGGGCCCCGGACCCGGACCCAGGCTCGGG GGGAAAGAGGAGGAGCCTGTCCGTCCTGGAGGGCCTGGGCTTCCGCGTGGGCCAGGCTCTGGGTGAGAGG CTGCCCCTGGAGACACCGGCCTTTAGGGAGGAGTTAGATGCCCTCAAGTTCCTGTGCAGAGATCTGTGGG CCGCCATGTTCCAGAAGCACATGGACGGACTCCGCACCAACCACCAGGGGACCTATGTGCTGCAGGACAA CAGCTTCCCCCTCCTTGTCACCATGGGCTCGGGGCCACAGTATCTAGAGGAAGCCCCCAAGTTCCTGGCC TTCACCTGCGGCCTTCTGTGCGGCGCCCTCCATACACTGGGCTTCCAGAGCCTGGTCACCGCCTCCGTGG CATCCCTGCCCGCCTGTAAGTTCCAGGTGGTGATCCAGAAGTCCTGAGGACCCCAGACCCCAGGCTTGCC CTTCCCTAAGCTTAGCCTCGGAATGTGGCACCTGACCCTGCCTCACTGCTCACCTTTGCAGGTCGCCTTG AAGCTGGAGCTCACAGGCTCTGGGGAGGTCACATGTGCTTCAGACAAGGGAATGAAAGGGCCGGGAGGGT CCCGGGAGGTGGGACCATCCCCTGAGTTCCAAGTCAGCATGGAGGGACATTAGGGCATCACCCAGATGAC AGATGTTCAGTAAAGGTTCTTTATGTGCAAACAGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:2811950 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:2811950 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6981 same embryo
	EMAGE:6986 same embryo
	EMAGE:6985 same embryo
	EMAGE:6984 same embryo
	EMAGE:6982 same embryo
	EurExpress:euxassay_007527 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source