EMAGE mouse gene expression spatial database

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EMAGE:7015

Fermt3 fermitin family homolog 3 (Drosophila) ( MGI:2147790)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002482. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002482_01	euxassay_002482_02	euxassay_002482_03	euxassay_002482_04

euxassay_002482_05	euxassay_002482_06	euxassay_002482_07	euxassay_002482_08	euxassay_002482_09

euxassay_002482_10	euxassay_002482_11	euxassay_002482_12	euxassay_002482_13	euxassay_002482_14

euxassay_002482_15	euxassay_002482_16	euxassay_002482_17	euxassay_002482_18	euxassay_002482_19

euxassay_002482_20	euxassay_002482_21	euxassay_002482_22	euxassay_002482_23	euxassay_002482_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7015_wholemount_strong.wlz

7015_wholemount_moderate.wlz

7015_wholemount_weak.wlz

7015_wholemount_possible.wlz

7015_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7015_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

weak

homogeneous

weak expression: see section 13 14 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5126
Entity Detected:	Fermt3, fermitin family homolog 3 (Drosophila) ( MGI:2147790)
Sequence:	sense strand is shown >T5126 CCACGCGTCCGCCAGCAACAGCCCGCCCTGGGGAGGGACTAGTACAACTTTCATGATAAGGCCACTACAG GCTGACCTCTGAAGGAAGCAACTGAGCAGGACCAGGCCAGACCAGGGTAACAGGTGTCCCACTGCCACCT GCAGCTGCAGCCCGTTGCAGGCTAGCAGAAACAGCCGCAGCCATGGCGGGTATGAAGACAGCCTCCGGGG ACTATATCGACTCTTCCTGGGAGCTGAGGGTGTTTGTGGGCGAGGAGGACCCTGAGGCCCAGTCTGTCAC ACTCCGAGTCACGGGGGAGTCGCACATTGGTGGGGTACTTCTGAAGATCGTGGAGGAAATCAATCGCAAG CAGGACTGGTCTGACCATGCCATTTGGTGGGAGCAGAAGAGACAGTGGCTGCTGCAGACACACTGGACGC TGGACAAGTACGGGATTCTGGCCGATGCCCGCCTCTTCTTTGGGCCACAGCACCGACCTGTCATCCTGCG ACTGCCCAACAGACGTGTGCTGCGGCTTCGGGCCAGTTTCTCCAAGCCCCTCTTCCAGACGGTGGCTGCC ATCTGCCGCCTCCTCAGTATCAGGCACCCAGAGGAGCTGTCTCTGCTGCGTGCTCCAGAGAAGAAGGAGA AGAAGAAGAAGGAGAAGGAGCCTGAAGAGGAGGTGCATGACCTGACAAAGGTTGTCTTAGCTGGCGGTGT GGCACCCACTTTATTCAGAGGCATGCCAGCACACTTCTCGGACAGCGCCCAGACTGAGGCTTGTTACCAC ATGCTGAGCCGGCCACAGCCGGCACCTGACCCTCTCCTGCTCCAGCGCCTGCCAAGGCCCAGCTCCCTGC CTGACAAGACCCAGCTCCACAGCAGGTGGCTGGATTCATCTCGGTGCCTCATGCAGCAAGGTATCAAGGC CGGGGATGTGCTTTGGCTGCGATTTAAGTACTACAGCTTTTTTGACCTGGATCCCAAGACAGACCCTGTG CGGCTGACCCAGCTGTATGAGCAGGCCCGCTGGGACTTACTGACAGAGGAGATCGACTGCACTGAAGAAG AGATGATGGTGTTTGCTGCCCTGCAGTACCACATCAACAAACTGACCCTGAGCGGGGATGTGGGTGAGCT GGCCTCTGGGGACCTGGGACTGGATGACCTGGATGCAGCCCTGAACAACCTGGAGGTGAAGCTGAAGGGG TCAGCACCCTCAGACATGCTGGATAGTCTCACTACCATCCCAGAACTCAAGGACCATCTCCGGATCTTCC GGCCCCGGAAGCTGACCCTGAAGGGCTACCGCCAGTACTGGGTGGTATTTAAGGACACCACACTGTCCTA CTATAAGAGCCAAGATGAAGCACCGGGGGACCCTACCCAGCAGCTCAACCTCAAGGGCTGTGAGGTGGTC CCTGATGTCAATGTCTCTGGCCAGAAGTTCTGCATCAAACTCCTGGTACCCTCACCAGAGGGCATGAGTG AGATCTACCTGAGGTGCCAGGATGAACAGCAGTACGCACAGTGGATGGCTGCCTGCCGACTGGCCTCCAA GGGCCGCACCATGGCGGACAGCAGCTATGCCAGCGAGGTGCAGGCCATTCTGGCCTTCCTCAGCCTGCAG CGGGCAGGTGGTAGCAATGGAGGCTCAGGGAACAAACCTCAAGGTCCTGAAGCCCCTGCTGAGGGCCTTA ACCCCTATGGCCTCGTGGCTCCTCGGTTCCAGCGAAAGTTCAAGGCCAAGCAGCTCACCCCAAGGATCCT GGAAGCTCACCAAAACGTGGCCCAACTCTCACTGACCGAGGCCCAGCTGCGCTTTATCCAGGCCTGGCAA TCCTTGCCCGACTTTGGCATCTCCTATGTGATGGTCAGGTTCAAGGGCAGCAGGAAAGATGAGATCCTAG GCATTGCCAACAACCGACTCATCCGGATTGATCTGGCCGTGGGTGACGTGGTCAAGACCTGGCGCTTCAG CAACATGCGGCAATGGAACGTCAACTGGGACATACGGCAGGTGGCCATTGAGTTCGATGAACACATCAAT GTGGCCTTCAGCTGTGTGTCTGCTAGCTGCCGCATCGTGCACGAGTACATCGGGGGTTACATCTTCCTGT CCACCCGAGAGCGGGCCCGAGGGGAAGAGCTGGATGAGGATCTGTTCCTGCAGCTTACAGGAGGCCATGA GGCCTTCTGAGGGCAGCCCCTGCTCCACTCACCACCTCCAGGGACCCCCCAAAGGCCACACCCACCTGAA CACACTGCTCCATCACTCCCGGGATGTACACTGCTGGGCAACTTCACTTGTTGTCACCAGGCAGGCCAGG GCCCTCGCTGGGGTAGGTGCTACCACCAGCCAGCCTAGGGATGATGGGCCCTGAGCCACACAGAGCCCCT CCTCTCACCCTGTTGGCAGAAGCTCACACCCCCGACCTCTATGTAGTCCCTAAGCACACGACGAATGCCA GACGAAGCACACGACGAATGCCAGACACGGATGAGGAACCACAAATCCTGGTTTGAAGCTGGACTTTCTT TCTGTGTCTGTTTCAGGTTTTTTAATAAATAAATATTTTATTGTTGAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5342463 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5342463 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7012 same embryo
	EMAGE:7016 same embryo
	EMAGE:7013 same embryo
	EMAGE:7017 same embryo
	EMAGE:7014 same embryo
	EurExpress:euxassay_002482 same experiment
	MGI:4824828 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source