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EMAGE:7015

Fermt3 fermitin family homolog 3 (Drosophila) ( MGI:2147790)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:7015 EMAGE:7015 EMAGE:7015 EMAGE:7015 EMAGE:7015
"Pseudo-wholemount" of euxassay_002482. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_002482_01 euxassay_002482_02 euxassay_002482_03 euxassay_002482_04
EMAGE:7015 EMAGE:7015 EMAGE:7015 EMAGE:7015 EMAGE:7015
euxassay_002482_05 euxassay_002482_06 euxassay_002482_07 euxassay_002482_08 euxassay_002482_09
EMAGE:7015 EMAGE:7015 EMAGE:7015 EMAGE:7015 EMAGE:7015
euxassay_002482_10 euxassay_002482_11 euxassay_002482_12 euxassay_002482_13 euxassay_002482_14
EMAGE:7015 EMAGE:7015 EMAGE:7015 EMAGE:7015 EMAGE:7015
euxassay_002482_15 euxassay_002482_16 euxassay_002482_17 euxassay_002482_18 euxassay_002482_19
EMAGE:7015 EMAGE:7015 EMAGE:7015 EMAGE:7015 EMAGE:7015
euxassay_002482_20 euxassay_002482_21 euxassay_002482_22 euxassay_002482_23 euxassay_002482_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:7015Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
7015_wholemount_strong.wlz
7015_wholemount_moderate.wlz
7015_wholemount_weak.wlz
7015_wholemount_possible.wlz
7015_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:7015_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
thymus primordium
weak weak
homogeneousweak expression: see section 13 14 15 16 17 18
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T5126
Entity Detected:Fermt3, fermitin family homolog 3 (Drosophila) ( MGI:2147790)
Sequence:sense strand is shown

>T5126
CCACGCGTCCGCCAGCAACAGCCCGCCCTGGGGAGGGACTAGTACAACTTTCATGATAAGGCCACTACAG
GCTGACCTCTGAAGGAAGCAACTGAGCAGGACCAGGCCAGACCAGGGTAACAGGTGTCCCACTGCCACCT
GCAGCTGCAGCCCGTTGCAGGCTAGCAGAAACAGCCGCAGCCATGGCGGGTATGAAGACAGCCTCCGGGG
ACTATATCGACTCTTCCTGGGAGCTGAGGGTGTTTGTGGGCGAGGAGGACCCTGAGGCCCAGTCTGTCAC
ACTCCGAGTCACGGGGGAGTCGCACATTGGTGGGGTACTTCTGAAGATCGTGGAGGAAATCAATCGCAAG
CAGGACTGGTCTGACCATGCCATTTGGTGGGAGCAGAAGAGACAGTGGCTGCTGCAGACACACTGGACGC
TGGACAAGTACGGGATTCTGGCCGATGCCCGCCTCTTCTTTGGGCCACAGCACCGACCTGTCATCCTGCG
ACTGCCCAACAGACGTGTGCTGCGGCTTCGGGCCAGTTTCTCCAAGCCCCTCTTCCAGACGGTGGCTGCC
ATCTGCCGCCTCCTCAGTATCAGGCACCCAGAGGAGCTGTCTCTGCTGCGTGCTCCAGAGAAGAAGGAGA
AGAAGAAGAAGGAGAAGGAGCCTGAAGAGGAGGTGCATGACCTGACAAAGGTTGTCTTAGCTGGCGGTGT
GGCACCCACTTTATTCAGAGGCATGCCAGCACACTTCTCGGACAGCGCCCAGACTGAGGCTTGTTACCAC
ATGCTGAGCCGGCCACAGCCGGCACCTGACCCTCTCCTGCTCCAGCGCCTGCCAAGGCCCAGCTCCCTGC
CTGACAAGACCCAGCTCCACAGCAGGTGGCTGGATTCATCTCGGTGCCTCATGCAGCAAGGTATCAAGGC
CGGGGATGTGCTTTGGCTGCGATTTAAGTACTACAGCTTTTTTGACCTGGATCCCAAGACAGACCCTGTG
CGGCTGACCCAGCTGTATGAGCAGGCCCGCTGGGACTTACTGACAGAGGAGATCGACTGCACTGAAGAAG
AGATGATGGTGTTTGCTGCCCTGCAGTACCACATCAACAAACTGACCCTGAGCGGGGATGTGGGTGAGCT
GGCCTCTGGGGACCTGGGACTGGATGACCTGGATGCAGCCCTGAACAACCTGGAGGTGAAGCTGAAGGGG
TCAGCACCCTCAGACATGCTGGATAGTCTCACTACCATCCCAGAACTCAAGGACCATCTCCGGATCTTCC
GGCCCCGGAAGCTGACCCTGAAGGGCTACCGCCAGTACTGGGTGGTATTTAAGGACACCACACTGTCCTA
CTATAAGAGCCAAGATGAAGCACCGGGGGACCCTACCCAGCAGCTCAACCTCAAGGGCTGTGAGGTGGTC
CCTGATGTCAATGTCTCTGGCCAGAAGTTCTGCATCAAACTCCTGGTACCCTCACCAGAGGGCATGAGTG
AGATCTACCTGAGGTGCCAGGATGAACAGCAGTACGCACAGTGGATGGCTGCCTGCCGACTGGCCTCCAA
GGGCCGCACCATGGCGGACAGCAGCTATGCCAGCGAGGTGCAGGCCATTCTGGCCTTCCTCAGCCTGCAG
CGGGCAGGTGGTAGCAATGGAGGCTCAGGGAACAAACCTCAAGGTCCTGAAGCCCCTGCTGAGGGCCTTA
ACCCCTATGGCCTCGTGGCTCCTCGGTTCCAGCGAAAGTTCAAGGCCAAGCAGCTCACCCCAAGGATCCT
GGAAGCTCACCAAAACGTGGCCCAACTCTCACTGACCGAGGCCCAGCTGCGCTTTATCCAGGCCTGGCAA
TCCTTGCCCGACTTTGGCATCTCCTATGTGATGGTCAGGTTCAAGGGCAGCAGGAAAGATGAGATCCTAG
GCATTGCCAACAACCGACTCATCCGGATTGATCTGGCCGTGGGTGACGTGGTCAAGACCTGGCGCTTCAG
CAACATGCGGCAATGGAACGTCAACTGGGACATACGGCAGGTGGCCATTGAGTTCGATGAACACATCAAT
GTGGCCTTCAGCTGTGTGTCTGCTAGCTGCCGCATCGTGCACGAGTACATCGGGGGTTACATCTTCCTGT
CCACCCGAGAGCGGGCCCGAGGGGAAGAGCTGGATGAGGATCTGTTCCTGCAGCTTACAGGAGGCCATGA
GGCCTTCTGAGGGCAGCCCCTGCTCCACTCACCACCTCCAGGGACCCCCCAAAGGCCACACCCACCTGAA
CACACTGCTCCATCACTCCCGGGATGTACACTGCTGGGCAACTTCACTTGTTGTCACCAGGCAGGCCAGG
GCCCTCGCTGGGGTAGGTGCTACCACCAGCCAGCCTAGGGATGATGGGCCCTGAGCCACACAGAGCCCCT
CCTCTCACCCTGTTGGCAGAAGCTCACACCCCCGACCTCTATGTAGTCCCTAAGCACACGACGAATGCCA
GACGAAGCACACGACGAATGCCAGACACGGATGAGGAACCACAAATCCTGGTTTGAAGCTGGACTTTCTT
TCTGTGTCTGTTTCAGGTTTTTTAATAAATAAATATTTTATTGTTGAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:5342463 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5342463 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:7012 same embryo
 EMAGE:7016 same embryo
 EMAGE:7013 same embryo
 EMAGE:7017 same embryo
 EMAGE:7014 same embryo
 EurExpress:euxassay_002482 same experiment
 MGI:4824828 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS