EMAGE mouse gene expression spatial database

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EMAGE:7033

Osbpl9 oxysterol binding protein-like 9 ( MGI:1923784)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002570. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002570_01	euxassay_002570_02	euxassay_002570_03	euxassay_002570_04

euxassay_002570_05	euxassay_002570_06	euxassay_002570_07	euxassay_002570_08	euxassay_002570_09

euxassay_002570_10	euxassay_002570_11	euxassay_002570_12	euxassay_002570_13	euxassay_002570_14

euxassay_002570_15	euxassay_002570_16	euxassay_002570_17	euxassay_002570_18	euxassay_002570_19

euxassay_002570_20	euxassay_002570_21	euxassay_002570_22	euxassay_002570_23	euxassay_002570_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7033_wholemount_strong.wlz

7033_wholemount_moderate.wlz

7033_wholemount_weak.wlz

7033_wholemount_possible.wlz

7033_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7033_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 17 18

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 04 05 06 07 17 18 19 20 21 weak expression: see section 16

spinal cord

moderate

homogeneous

moderate expression: see section 09 10 11 12 13 14 15 16 17

cervico-thoracic ganglion

weak

regional

weak expression: see section 08 15

cervical ganglion

weak

regional

weak expression: see section 07 08 15 16

dorsal root ganglion

moderate

regional

moderate expression: see section 09 10 11 16 17 18 weak expression: see section 07 08 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5174
Entity Detected:	Osbpl9, oxysterol binding protein-like 9 ( MGI:1923784)
Sequence:	sense strand is shown >T5174 CCACGCGTCCGCGGACGCGTGGGCCCCAGTGTCCAAGACTTTGATAAGAAACTTACCGAGGCTGACGCGT ACCTGCAGATCTTGATAGAACAATTAAAGCTTTTTGATGACAAGCTTCAAAATTGTAAAGATGATGAACA GAGAAAGAAAGTTGAAACTCTCAAAGACACAACAAATAGCATGGTAGAATCAATTAAACACTGCATTGTG TTGCTACAGATTGCTAAAAGTACTATTAATCCTGTAGATGCAATATACCAGCCTAGTCCCTTGGAACCTG TGATCAGCACAATGCCTTCCCAGACTGCCTTACCTCCAGAACCCGCTCAGTTGTGTAAGTCAGAGCAGCG TCCATCTTCCTTACCTGTTGGACCTGTGTTAGCTACCTTGGGACATCATCAGACTCCAACACCAAATAGT ACAGGCAGTGGGAACTCACCACCTAGCAGCAGTCTGACTCCTCCCAGCCATGTCAACTTGTCTCCAAATA CAGTCCCAGAGTTCTCTTACTCTAGCAGTGAAGATGAGTTCTATGATGCTGATGAATTCCATCAAAGTGG CTCGTCCCCAAAGCGCTTAATAGATTCTTCTGGATCTGCCTCAGTCTTGACACACAGCAGCTCCGGAAAT AGCTTAAAACGCCCAGATACCACAGAGTCTCTGAATTCCTCCATGTCCAATGGCACAAGCGATGCTGATC TTTTTGACTCACATGACGACAGAGATGATGATGGGGAGGCTGGGTCAGTGGAGGAGCACAAGAGCGTTAT CATGCACCTCTTATCACAAGTCAGGCTGGGGATGGACCTCACAAAGGTAGTTCTTCCAACGTTTATTCTC GAGAGAAGATCTCTGTTAGAAATGTATGCAGACTTTTTCGCACATCCAGACCTGTTCGTGAGCATTAGTG ATCAGAAGGATCCCAGGGATCGAATGGTTCAGGTTGTGAAATGGTACCTCTCGGCCTTCCATGCAGGAAG GAGAGGATCGGTGGCCAAAAAGCCGTACAATCCTATTTTGGGTGAGATCTTTCAGTGTCACTGGACGTTG CCGAATGATACTGAAGAGAACGCAGAGCTCGTTTCAGAAGGGCCGGTTCCCTGGGTTTCTAAGAACAGTG TAACATTTGTGGCTGAGCAAGTTTCCCACCATCCGCCCATTTCAGCCTTTTATGCTGAGTGTTTTAACAA GAAGATACAATTCAATGCTCATATCTGGACTAAATCAAAATTCCTTGGGATGTCAATTGGGGTACACAAC ATAGGTCAGGGCTGTGTCTCGTGTCTGGAGTACGATGAGCACTACATCCTCACGTTCCCCAATGGCTATG GAAGGTCTATCCTGACAGTGCCCTGGGTGGAATTGGGAGGAGAATGCAATATCAACTGCTCCAAAACGGG TTACAGCGCAAACATCGTCTTCCACACTAAGCCTTTCTATGGGGGCAAGAAGCACAGAATTACTGCAGAG ATTTTTTCTCCGAATGACAAGAAATCCTTCTGCTCAATTGAAGGGGAATGGAATGGTATCATGTATGCAA AATACGCAACAGGGGAAAACACTGTCTTTGTAGACACCAAGAAATTGCCTATAATCAAGAAAAAGGTGAG GAAGTTGGAAGATCAGAATGAGTATGAGTCCCGCAGCCTTTGGAAGGATGTCACTTTCAATTTAAAAATC AGAGACATTGATGCAGCAACGGAAGCAAAGCACAGACTTGAAGAAAGACAAAGAGCAGAAGCCCGAGAAA GGAAGGAGAAGGAAATTCAGTGGGAGACGAGGCTCTTTCACGAGGATGGCGAATGCTGGGTTTACGATGA GCCTTTACTGAAGCGTCTTGGTGCTGTGAAGCATTAGGCCGACAACCGAGTCCACACCTGGTGACCAGGG CAGTAGGCGTAATTAAGCAACAATCGATCTTCCTTCAGGAGAGCTTGTCACTTCCTTCTTAACGCAGTGG TTCCTATCTCAGGGATACTGGACTTGACGACACAGATGAACAATTAAAGTGGAAACCGCTTCCCTTTTCT CCTCTGTGGCAGTTACAATTTTGACTTCAGGCCTGAGAAAAACTTCAGGTTTCGAAACATGACATCTCTT CCTTTTCCAGATCCCATGCTTTGAAAAATATTTATAGACAGTTCCAGGTCTCAGCTTCCTGTCCTCTAGT TCTGCTGTTCGGGCATAAAATCTTTATCTCCAGTTCATATAATCTTGAGTTTTAGATATACACACATGCG TAACAGCTGACAGTTTTTCACAAGTACACCCACCTGTAAATACTGTATCCTCAGTTTAGAAAATTAGTGC AATGTATGAAAATCAAGTGTTAGGAAATTTCATGGTTTCACCTATAACCTTTATTTTAGAATTGAACTAT GATTAGATTGTATCTAAACCTGAAGTATAATTATATGCAGTGCTTCTTAAGGCTTCATAAAATAATTTTC CAACCTTTTTAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5347121 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5347121 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7030 same embryo
	EMAGE:7032 same embryo
	EMAGE:7035 same embryo
	EMAGE:7034 same embryo
	EMAGE:7031 same embryo
	EurExpress:euxassay_002570 same experiment
	MGI:4826993 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source