EMAGE mouse gene expression spatial database

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EMAGE:7035

Klhl28 kelch-like 28 (Drosophila) ( MGI:1913939)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002559. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002559_01	euxassay_002559_02	euxassay_002559_03	euxassay_002559_04

euxassay_002559_05	euxassay_002559_06	euxassay_002559_07	euxassay_002559_08	euxassay_002559_09

euxassay_002559_10	euxassay_002559_11	euxassay_002559_12	euxassay_002559_13	euxassay_002559_14

euxassay_002559_15	euxassay_002559_16	euxassay_002559_17	euxassay_002559_18	euxassay_002559_19

euxassay_002559_20	euxassay_002559_21	euxassay_002559_22	euxassay_002559_23	euxassay_002559_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7035_wholemount_strong.wlz

7035_wholemount_moderate.wlz

7035_wholemount_weak.wlz

7035_wholemount_possible.wlz

7035_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7035_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

facial vii ganglion

moderate

regional

moderate expression: see section 21

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 17 18

trigeminal v ganglion

moderate

regional

moderate expression: see section 02 03 04 05 06 07 15 16 17 18 19 20 21

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 06 07 18

cervico-thoracic ganglion

weak

regional

weak expression: see section 08 15

cervical ganglion

weak

regional

weak expression: see section 07 08 15 16

dorsal root ganglion

moderate

regional

moderate expression: see section 07 08 09 10 15 16 17

not examined

regional

not examined expression: see section 01 02 21 22 23

neural retina

weak

regional

weak expression: see section 01 02 21 22 23

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 07 08 09 10 12 13 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5175
Entity Detected:	Klhl28, kelch-like 28 (Drosophila) ( MGI:1913939)
Sequence:	sense strand is shown >T5175 CCACGCGTCCGGTTTGACGGGAGCAAGAGAGATCCCATCCCGGGTTCCAGTTTTCCGTGGCTCCTCCTCC TGTTGCTGCAGGTGTTATTCCTGGGACTCAGATGGACCACACAGCCCCGACCTACATGCTTGCTAACTTA ACCCACTTACATTCTGAACAACTTCTGCAGGGATTGAATCTTCTTCGCCAGCATCACGAACTCTGTGACA TCATTCTTCGAGTAGGTGATGTTAAAATTCATGCTCATAAAGTGGTCCTTGCCAGCATCAGCCCATACTT CAAAGCTATGTTCACTGGCAACCTTTCTGAGAAAGAGAACAGTGAGGTTGAGTTTCAGTGCATCGATGAA GCTGCGCTGCAGGCCATTGTGGAGTACGCCTATACGGGCACGGTTTTCATTTCTCAGGACACAGTTGAAT CTCTCCTGCCAGCAGCAAACTTACTCCAGATAAAACTCGTTCTTAAAGAATGCTGTGCATTTCTTGAAAG CCAACTTGACCCTGGTAATTGTATTGGAATTTCTCGTTTTGCGGAGACTTACGGTTGTCATGACCTTTAT TTGGCGGCTACTAAATTCATATGCCAGAATTTTGAATCTGTTTGCCAGACAGAAGAGTTTTTTGAGCTTA CACATGCTGATTTGGACGAAATTGTCTCCAATGACTGCTTGAATGTGGCTACTGAAGAGACAGTTTTTTA TGCATTAGAATCTTGGATCAAGTACGATGTACAAGAACGGCAGAAGTACTTAGCCCAGCTGCTGAACAGT GTACGGTTACCATTGCTGAGCGTTAAGTTCCTCACTAGGCTCTATGAAGCGAATCATCTTATCCGTGATG ATCGCACCTGTAAACATCTCTTGAATGAAGCCCTAAAGTACCACTTCATGCCTGAACATAGACTCTCTCA TCAGACAGTCTTGATGACACGACCTCGCTGTGCTCCCAAAGTACTGTGTGCAGTAGGAGGAAAATCTGGA CTCTTTGCCTGTTTGGATAGTGTGGAGATGTACTTTCCCCAGAATGACTCTTGGATTGGTCTGGCGCCCT TAAATATTCCTCGCTATGAATTCGGAATATGTGTTTTAGACCAAAAAGTCTTTGTTATAGGTGGTATTGA AACTAGCGTGCGTCCTGGTATGACTGTCAGAAAACATGAAAATTCAGTGGAATGTTGGAATCCTGATACA AACACCTGGACTTCTCTCGAGAGAATGAACGAGAGCCGGAGTACTCTCGGAGTGGCAGTGCTCGCGGGAG AAGTTTTTGCCTTAGGTGGATATGACGGGCAGTCTTACTTGCAATCTGTAGAAAAATACATCCCCAAAAT AAGGCAGTGGCAACCTGTGGCCCCAATGACAACCACAAGAAGTTGTTTTGCTGCAGCTGTCCTGGATGGA ATGCTATACGCCATTGGAGGCTATGGCCCCGCTCACATGAACAGTGTGGAGCGTTACGATCCGAGTAAGG ACTCCTGGGAGATGGTTGCGCCCATGGCTGATAAGAGGATTCACTTTGGCGTCGGAGTCATGCTAGGTTT TATTTTCGTGGTAGGTGGGCATAACGGTGTCTCACACTTGTCAAGCATTGAAAGATATGATCCTCATCAA AATCAGTGGACTGTGTGTAGACCAATGAAAGAGCCCAGGACAGGAGTTGGTGCTGCAGTAATCGATAACT ACCTGTATGTAGTCGGTGGTCACTCAGGGTCTTCCTATCTGAACACAGTACAGAAATACGACCCTATCTC AGATACATGGCTGGATTCAGCAGGCATGATATACTGTCGCTGCAATTTTGGATTAACTGCACTTTGACAA GTGTGAACTTGTGGAAATCGCACAAGGAGGTACTACAGGAAAGAGTGCCCAGTGTCCTGAACCTGACAGC TAGCTGCACTGCTTTCTTTGTAACGTAAAGTGACATGAAGACTCAGTTTTGTTGGGTACTTTGAACTGAC TAATAGCTTGCTCTTGATTATATAGTGAATTAATAATTAAACAAGGGAAAAAAAAAAAAAAAAAAAAAAA AA
Notes:	The probe template was PCR amplified from IMAGE:5347183 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5347183 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7030 same embryo
	EMAGE:7032 same embryo
	EMAGE:7034 same embryo
	EMAGE:7031 same embryo
	EMAGE:7033 same embryo
	EurExpress:euxassay_002559 same experiment
	MGI:4825799 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source