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EMAGE:7065

Slc6a20a solute carrier family 6 (neurotransmitter transporter), member 20A ( MGI:2143217)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:7065 EMAGE:7065 EMAGE:7065 EMAGE:7065 EMAGE:7065
"Pseudo-wholemount" of euxassay_002422. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_002422_01 euxassay_002422_02 euxassay_002422_03 euxassay_002422_04
EMAGE:7065 EMAGE:7065 EMAGE:7065 EMAGE:7065 EMAGE:7065
euxassay_002422_05 euxassay_002422_06 euxassay_002422_07 euxassay_002422_08 euxassay_002422_09
EMAGE:7065 EMAGE:7065 EMAGE:7065 EMAGE:7065 EMAGE:7065
euxassay_002422_10 euxassay_002422_11 euxassay_002422_12 euxassay_002422_13 euxassay_002422_14
EMAGE:7065 EMAGE:7065 EMAGE:7065 EMAGE:7065 EMAGE:7065
euxassay_002422_15 euxassay_002422_16 euxassay_002422_17 euxassay_002422_18 euxassay_002422_19
EMAGE:7065 EMAGE:7065 EMAGE:7065 EMAGE:7065
euxassay_002422_20 euxassay_002422_21 euxassay_002422_22 euxassay_002422_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:7065Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
7065_wholemount_strong.wlz
7065_wholemount_moderate.wlz
7065_wholemount_weak.wlz
7065_wholemount_possible.wlz
7065_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:7065_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T2087
Entity Detected:Slc6a20a, solute carrier family 6 (neurotransmitter transporter), member 20A ( MGI:2143217)
Sequence:sense strand is shown

>T2087
TGGCCTCGAGGCAAGAATTCGGATCCATGAGCAACCACACAGGCTACGATGAGGAGTGTGAGAAGGCTTC
GTCGACACAGTACTTCTGGTACAGGAAAACACTCAACATCTCACCGTCCATCCAGGAGAATGGAGGAGTG
CAGTGGGAGCCAGCGCTGTGCCTCACCCTGGCCTGGCTGATGGTCTATCTGTGCATCCTGAGAGGCACCG
AATCTACTGGCAAGGTGGTCTACTTCACCGCATCGATGCCTTACTGTGTTCTTATTATCTACTTGGTCCG
TGGCCTTACACTCCATGGAGCCACCAATGGCCTGATGTACATGTTCACACCTAAGATGGAACAGCTAGCC
AACCCCAAGGCCTGGATCAATGCAGCCACGCAGATCTTCTTCTCACTGGGCTTGGGTTTTGGCAGCCTGA
TCGCTTTTGCCAGCTACAATGAACCCTCCAACAACTGCCAGAAGCATGCCATTATTGTGTCCATCATCAA
CAGCTCCACCTCCATATTTGCCAGCATTGTCACCTTCTCCATCTATGGCTTCAAGGCCACCTTCAACTAT
GAA
Notes:The probe template was PCR amplified from IMAGE:848681 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:848681 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:7061 same embryo
 EMAGE:7062 same embryo
 EMAGE:7064 same embryo
 EMAGE:7063 same embryo
 EMAGE:7060 same embryo
 EurExpress:euxassay_002422 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS