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EMAGE:710

Myod1 myogenic differentiation 1 ( MGI:97275)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:710
Fig 1E Goldhamer et al, 1995 [PMID:7720572] . Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Staining of the otic vesicle is non-specific background. Image annotation: ov= otic vesicle.
Expression Pattern Description
Spatial Annotation:
EMAGE:710Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
710_wholemount_strong_3D_1.wlz
710_wholemount_moderate_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:710_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
branchial arch
weak weak
Low levels of expression are not apparent in this preparation
forelimb bud
weak weak
Low levels of expression are not apparent in this preparation
trunk somite
detected detected
regionalExpression is in the ventral region of the myotomes of the thoracic somites (white arrow). Expression is most abundant in the dorsal region of the anterior somites
Annotation Validation: Submitter + EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1309772
Entity Detected:Myod1, myogenic differentiation 1 ( MGI:97275)
Sequence:sense strand is shown

>MGI:1309772
GCGTCCAGCCCGCGCTCCAACTGCTCTGATGGCATGATGGATTACAGCGGCCCCCCAAGCGGCCCCCGGC
GGCAGAATGGCTACGACACCGCCTACTACAGTGAGGCGGTGCGCGAGTCCAGGCCAGGGAAGAGTGCGGC
TGTGTCGAGCCTCGACTGCCTGTCCAGCATAGTGGAGCGCATCTCCACAGACAGCCCCGCTGCGCCTGCG
CTGCTTTTGGCAGATGCACCACCAGAGTCGCCTCCGGGTCCGCCAGAGGGGGCATCCCTAAGCGACACAG
AACAGGGAACCCAGACCCCGTCTCCCGACGCCGCCCCTCAGTGTCCTGCAGGCTCAAACCCCAATGCGAT
TTATCAGGTGCTTTGAGAGATCGACTGCAGCAGCAGAGGGCGCACCACCGTAGGCACTCCTGGGGATGGT
GCCCCTGGTTCTTCACGCCCAAAAGATGAAGCTTAAATGACACTCTTCCCAACTGTCCTTTCGAAGCCGT
TCTTCCAGAGGGAAGGGAAGAGCAGAAGTCTGTCCTAGATCCAGCCCCAAAGAAAGGACATAGTCCTTTT
TGTTGTTGTTGTTGTAGTCCTTCAGTTGTTTGTTTGTTTTTTCATGCGGCTCACAGCGAAGGCCACTTGC
ACTCTGGCTGCACCTCACTGGGCCAGAGCTGATCCTTGAGTGGCCAGGCGCTCTTCCTTTCCTCATAGCA
CAGGGGTGAGCCTTGCACACCTAAGCCCTGCCCTCCACATCCTTTTGTTTGTCACTTTCTGGAGCCCTCC
TGGCACCCACTTTTCCCCACAGCTTGCGGAGGCCACTCAGGTCTCAGGTGTAACAGGTGTAACCATACCC
CACTCTCCCCCTTCCCGCGGTTCAGGACCACTTATTTTTTTATATAAGACTTTTGTAATCTATTCGTGTA
AATAAGAGTTGCTTGGCCAGAGCGGGAGCCCCTTGGGCTATATTTATCTCCCAGGCATGCTGTGTAGTGC
AACAAAAACTTTGTATGTTTTATTCCTCAAGCGGGCGAGTCAGGTGTTGGAAATAA
nt 783 - nt 1818 of NM_010866.1
Notes:The Myod1 (MyoD) probe used in this study by Goldhamer et al, 1995 [PMID:7720572] is described as "nucleotides 751 to 1785 of the mouse myoD cDNA (Sassoon et al., 1989 [PMID:2552320] ". Sassoon et al describe the probe as nt 751-1785 of the sequence shown in Fig8 of Davis et al [PMID:3690668] .
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:FVB/N
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Goldhamer et al, 1995 [PMID:7720572] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:7720572] Goldhamer DJ, Brunk BP, Faerman A, King A, Shani M, Emerson CP 1995 Embryonic activation of the myoD gene is regulated by a highly conserved distal control element. Development (121):637-49
 [ doi:10.1038/341303a0] [ PMID:2552320] Sassoon D, Lyons G, Wright WE, Lin V, Lassar A, Weintraub H, Buckingham M 1989 Expression of two myogenic regulatory factors myogenin and MyoD1 during mouse embryogenesis. Nature (341):303-7
 [ doi:10.1016/0092-8674(87)90585-X] [ PMID:3690668] Davis RL, Weintraub H, Lassar AB 1987 Expression of a single transfected cDNA converts fibroblasts to myoblasts. Cell (51):987-1000
Links:MGI:1334370 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI