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EMAGE:7132

3010026O09Rik RIKEN cDNA 3010026O09 gene ( MGI:1915317)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:7132 EMAGE:7132 EMAGE:7132 EMAGE:7132 EMAGE:7132
"Pseudo-wholemount" of euxassay_002646. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_002646_01 euxassay_002646_02 euxassay_002646_03 euxassay_002646_04
EMAGE:7132 EMAGE:7132 EMAGE:7132 EMAGE:7132 EMAGE:7132
euxassay_002646_05 euxassay_002646_06 euxassay_002646_07 euxassay_002646_08 euxassay_002646_09
EMAGE:7132 EMAGE:7132 EMAGE:7132 EMAGE:7132 EMAGE:7132
euxassay_002646_10 euxassay_002646_11 euxassay_002646_12 euxassay_002646_13 euxassay_002646_14
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euxassay_002646_15 euxassay_002646_16 euxassay_002646_17 euxassay_002646_18 euxassay_002646_19
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euxassay_002646_20 euxassay_002646_21 euxassay_002646_22 euxassay_002646_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:7132Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
7132_wholemount_strong.wlz
7132_wholemount_moderate.wlz
7132_wholemount_weak.wlz
7132_wholemount_possible.wlz
7132_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:7132_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T3366
Entity Detected:3010026O09Rik, RIKEN cDNA 3010026O09 gene ( MGI:1915317)
Sequence:sense strand is shown

>T3366
GCCGGCTCAGCAGTCTCGGGTTCTACGGTGCTGCAGCTGCCACATCTTTCAGGCGCATCAGGTGAAGAAG
AGTCTCAAATGGACATGCAAGGCTTGTGGAGAGAAGCAGTCATTTGTTCGGGCTTACGGTGAAGGCTCTG
GTGCTGACTGCAGACGCCATGTCCAAAAGCTAAATCTGCTGCAAGGACAAGTGTCAGAGCTGTCACTCAG
GTCTGTGGAGGAAGCTGTAAATGGCAGTGAAGAAGAAAATGCAGGCCCTCTGCAGGCCGAGGCTGGGAGT
CAGCAGGCACCGTCTAAGCCCCTCGAGTCCCGTTGGTTGAAGTATCTGGACAAGGGCTGCGAGGACCAGG
AGCTGGACAGAGGGAGACCAGCTCTTAAGACACAGCTTTCAACCAGTGCTGAGCGGCCAAGCAGTCCAGC
CCAACCCAGGAAGAGGAAATGGAATCAGCGCACAGGGCAGCCTGCACACAGCCTGCATGGCCAGGGTGTG
GACAGTGTTGAAGACAACTTTGAACACCAGGA
Notes:The probe template was PCR amplified from IMAGE:2936849 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2936849 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:7127 same embryo
 EMAGE:7131 same embryo
 EMAGE:7128 same embryo
 EMAGE:7130 same embryo
 EMAGE:7129 same embryo
 EurExpress:euxassay_002646 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS