EMAGE mouse gene expression spatial database

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EMAGE:7145

Tdrd3 tudor domain containing 3 ( MGI:2444023)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004569. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004569_01	euxassay_004569_02	euxassay_004569_03	euxassay_004569_04

euxassay_004569_05	euxassay_004569_06	euxassay_004569_07	euxassay_004569_08	euxassay_004569_09

euxassay_004569_10	euxassay_004569_11	euxassay_004569_12	euxassay_004569_13	euxassay_004569_14

euxassay_004569_15	euxassay_004569_16	euxassay_004569_17	euxassay_004569_18	euxassay_004569_19

euxassay_004569_20	euxassay_004569_21	euxassay_004569_22	euxassay_004569_23	euxassay_004569_24

euxassay_004569_25

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7145_wholemount_strong.wlz

7145_wholemount_moderate.wlz

7145_wholemount_weak.wlz

7145_wholemount_possible.wlz

7145_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7145_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5828
Entity Detected:	Tdrd3, tudor domain containing 3 ( MGI:2444023)
Sequence:	sense strand is shown >T5828 TTTTTCTTTTCTTTCTTCTTTTTTTCCCCCTCTTTCTTCTTTTTCTTTTTTCTGGGTCTCCAGTAGGAGG CCCGTCCCCCACCCTCAGTTCAGACCCCCGCCGTCCCCGAGCCTGAGCAGCTAACCATGGCCGAGGTGTC CGGCGCCGCGTTGTCCCAGGCGGGTTGGTACCTTTCAGATGAAGGCGTTGAAGCTTGCACAAGCTCTCCT GGGAAAGGCAGCATAAATGACATCATCCTGATTGCTCTCAATACAGATTTGAGAACAATTGGCAAGAAGT TCCTTCCCAGTGACATCAACGGTGGAAAGGTAGAGAAGCTTGAAGGTCCATGTGTCTTACAAATTCAGAA GGTCCGCAATGTTGCTGCGCCAAAGGATAACGAGGAATCGCAAGCTGCGCCAAGGATGCTCCGCGTGCAG ATGACGGATGGGCATACAAGTTGTACGGCAGTAGAATTCAGTTATATTTCAAAAATAAGCCTGAACACAC CACCTGGCACAAAAGTGAAGCTCTCTGGCACTGTGGACATAAAGAACGGATTCCTGCTCTTAAGTGACTC TAACACCACAGTTCTTGGTGGTGAAGTGGAACACCTAATTGACAAGTGGGCTTTACAGAGAAGCTTATTA AAGCATAACAGAAGCAATATTGGAGCTGAAGGTGGCCCACCTCCTTTTCTACCTTTTGGGCAGAAGTGTG CATCTAATGTCCAAGTGGATAGCAGAGAGCTTGATCGAAGAAAGACATTACAAGTTTCCCTGCCTGCCAA GCCTGCAAATGATAATGATGAATTTGAAAAGCAAAGAACTGCTGCCATTGCAGAGGTTGCAAAGAGCAAA GAGACCAAGACATTTGGAGGAGGTGGAGGTGGTGCCAGAAGTAATCTCAATATTGGTGCTGCTGGTCACC GAAATAGGGAGGTTTTACAGAAGGAAAAGGCTTCCAAGTCAGAGAGCAAGAACGAAGGTGTCTATAGAGA ATTGGTTGATGAGAAAGCCCTGAAACACATAACAGAAATGGGCTTCAGTAAGGAGGCTTCGAGGCAAGCG CTGATGGATAATGCCAACAACTTAGAAGCAGCACTGAATGTCCTTCTGAACAGCAGTAAACAGAAGCCTG CTGTGGGCCCTCCTGCTAGAGGAAGAGGGAAAGGCAGGGGTCGAGGACGATCAGAAGACGAGGAAGACCT GGGAACCGCAAGACCATCAGCACCAAGCACGTTATTTGATTTCTTGGAGTCTAAAATGGGAACTCTGAAT GTGGAAGAACCTAAGTCACAGCCACAGCACCTTCATCAAGGACAGCACAGAGGATGGAATGCCGAGCAAA ATGGGATGAAGGACGGCAGTCAGTCAAGACATCTTCCTCGAAACGATACCAGGCAGCCAAGAAATGAGAG GCCACCTCGCTTTCAAAAAGACACCCCAACTTCCAAGTCAACTGTAGAAAACAGCGTATTATCTAGAAAT AGAGGCTCCGAAAGGCCGAGTAGTTCCTCAGGTTCTGATGTGTGGGCAGAAGAGAGGATCAGGTGTGACA GGCCATATTCTAGGTATGACAGAACTAAAGACGCTTCACATCCTCTAGGCTTTCAGCACAACGATGGTGC TTTTAAAAAGAGAGAGAACTCTATGCAAAACAGACCAGGAAGAGGCCCTTTGTATGCAGAGGCCAAAGAA AACCCACTTCCTCCAGAATTCGTAGATTACAATAATCAAAGACGTGGGAGAAGAGAAAACCAAACAGGTC ATCCTGATCACTGTTATGAGAGGAAACCACGGACAATGAATAGTGAAGCTTTTAGTGGTCTAAAAATTGA GAAACATTTTAGTGTAAACACTGATTACCCGAGTCCTGTGCAGAGCAATAGTCTTGGTGTTCCAAATGGA GAGACAGCTCCGCCTCTGAAAGGAAGGCGCGTAGGACCGATTAAGTCAGCAGGACCTGTCACAGCTGTGC CCTACGATGATAAAATATTTTATAATAGTGGGCCCAAAAGAAGGTCTGGGCCAATTAAGCCTGAAAAGGT GATAGAATCATCTATTCCTGTGGAGTATGCAAAAGTGTGGAAACCTGGCGATGAATGTTTTGCACTTTAT TGGGAAGACAACAAGTTTTACCGAGCAGAAGTTGAAGCCTTGCATTCTTCGGGTATGACAGCGGTCGTTA AGTTCACGGACTACGGCAACTATGAAGAGGTGCTACTGAGCAATATCAAGCCTGTTCAGACAGAGGCATG GGAAGAAGAAGGCACCTATGATCACACCATTGAGTTTCGCAGAGGAGGTGATGGACAGCCACGAAGGTCT ACTCGGCCAACGCAGCAGTTTTACCAACCTCCTCGAGCTCGGAACTAATGTGAAAGGGCTTTTGTGAAGG AAGAGCCACTGACTGAAACATCCTGGAAGCCCACTGATAGATTTTCCACTTCTTCAGAGAGAGACTCAAT ATGCTGGATATGATTTTTGGAGAAAGAAAAAAGAGGCAGATATTAGGGAAACGATTCAATTTACACAAGG CAATGGGGGGAAATACTGAGTTCGATAGAGCAAAATATCTTTACAAGTGGAAAACTTCTGCCATCAATAA AAGCATTGCATTGTTATATAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3153274 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3153274 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7147 same embryo
	EMAGE:7150 same embryo
	EMAGE:7146 same embryo
	EMAGE:7149 same embryo
	EMAGE:7148 same embryo
	EurExpress:euxassay_004569 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source