EMAGE mouse gene expression spatial database

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EMAGE:7146

Tmem208 transmembrane protein 208 ( MGI:1913570)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004557. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004557_01	euxassay_004557_02	euxassay_004557_03	euxassay_004557_04

euxassay_004557_05	euxassay_004557_06	euxassay_004557_07	euxassay_004557_08	euxassay_004557_09

euxassay_004557_10	euxassay_004557_11	euxassay_004557_12	euxassay_004557_13	euxassay_004557_14

euxassay_004557_15	euxassay_004557_16	euxassay_004557_17	euxassay_004557_18	euxassay_004557_19

euxassay_004557_20	euxassay_004557_21	euxassay_004557_22	euxassay_004557_23	euxassay_004557_24

euxassay_004557_25	euxassay_004557_26	euxassay_004557_27	euxassay_004557_28

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7146_wholemount_strong.wlz

7146_wholemount_moderate.wlz

7146_wholemount_weak.wlz

7146_wholemount_possible.wlz

7146_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7146_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5824
Entity Detected:	Tmem208, transmembrane protein 208 ( MGI:1913570)
Sequence:	sense strand is shown >T5824 TACTGACTGCAGTTCCAGTTACTCGCCCTTGTTTCAGTGGCTGGGTGTCTGCTGCGCACCATGGCGCCCA AAGGAAAAGTGGGTACCAGAGGGAAGAAACAGATATTTGAAGAGAACAAAGAAACTCTCAAGTTTTACCT TCGGATCATACTGGGAGCCAACGCCATCTACTGCCTTGTAACCTTGGTCTTCTTCTATTCCTCTGCCTCA TTCTGGGCCTGGATGGCCCTGGGCTTTAGCTTGGCCGTGTACGGGGCCAGCTACCACTCTATGAGCTCGA TGGCTCGGGCATCCTTCTCTGAGGATGGGTCCTTGATGGATGGTGGCATGGACCTCAACATGGAGCAGGG CATGGCAGAGCACCTTAAGGATGTGATCCTACTGACAGCCATTGTTCAGGTGCTCAGCTGTTTCTCCCTC TACATCTGGTCCTTCTGGCTTCTGGCTCCAGGCCGGGCCCTTTACCTTTTGTGGGTAAACGTGCTGGGCC CCTGGTTCACAGCAGACAGTGGAGCCCCCTGCACCAGAGCTCAATGAGAAACGACAACGCCGACAGGAGC GGCGGCAGATGAAAAGGTTATAGCATCCTTAACTGAGGCTGTAGGCTGCTGGGCACTGGTTGGCTCTGTT ATGCTGCGTGGCCCCATGCCTGGAGCAGTGAGGGCCTAGTTTAAGGGCCAAAGCAGCCTGGATTATAGGA TCACTAAATAAATAGTTTCGAATGTGGCTAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3152664 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3152664 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7147 same embryo
	EMAGE:7150 same embryo
	EMAGE:7149 same embryo
	EMAGE:7148 same embryo
	EMAGE:7145 same embryo
	EurExpress:euxassay_004557 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source