EMAGE mouse gene expression spatial database

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EMAGE:7148

Slc38a4 solute carrier family 38, member 4 ( MGI:1916604)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004567. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004567_01	euxassay_004567_02	euxassay_004567_03	euxassay_004567_04

euxassay_004567_05	euxassay_004567_06	euxassay_004567_07	euxassay_004567_08	euxassay_004567_09

euxassay_004567_10	euxassay_004567_11	euxassay_004567_12	euxassay_004567_13	euxassay_004567_14

euxassay_004567_15	euxassay_004567_16	euxassay_004567_17	euxassay_004567_18	euxassay_004567_19

euxassay_004567_20	euxassay_004567_21	euxassay_004567_22	euxassay_004567_23	euxassay_004567_24

euxassay_004567_25

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7148_wholemount_strong.wlz

7148_wholemount_moderate.wlz

7148_wholemount_weak.wlz

7148_wholemount_possible.wlz

7148_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7148_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7424
Entity Detected:	Slc38a4, solute carrier family 38, member 4 ( MGI:1916604)
Sequence:	sense strand is shown >T7424 TGAATGTTTTTATCCCCATGGAAAGGGCTGTGAGGTCAAATGGACCCCATGGAACTGAACAACGTCAGCA TCGAACCCGACGGAGACAGCTGCAGCGGGGACAGTATTCAGGACAGCTACACCGGCATGGAAAACTCCGA CAAGGACGCCATGAACAGCCAATTTGCTAATGAAGATGCCGAAAGTCAGAAGTTCCTGACAAATGGGTTT TTAGGGAAGAAGAAGCTAGCCGATTACGCGGATGAGCATCACCCTGGAATGACTTCCTTTGGAATGTCCT CATTTAACCTGAGCAACGCCATCATGGGCAGTGGGATCTTAGGCTTGTCCTATGCCATGGCCAACACCGG GATCATCCTTTTTATAATCATGCTGCTTACTGTGGCAATACTCTCGCTCTACTCGGTTCACCTTTTGCTG AAGACAGCCAAGGAAGGAGGGTCTCTAATCTATGAAAAATTGGGCGAGAAAGCATTTGGATGGCCTGGGA AAATTGGAGCCTTCATCTCTATTACAATGCAGAACATTGGAGCCATGTCAAGCTACCTCTTCATCATTAA GTACGAACTGCCTGAAGTAATCAGAGCATTCATGGGACTTGAAGAAAACACTGGGGAATGGTACCTCAAC GGCAACTACCTCGTCTTATTTGTGTCCGTGGGGATCATCCTCCCGCTCTCTCTCCTTAAAAATTTAGGCT ACCTTGGCTACACCAGTGGATTTTCTCTCTCCTGCATGGTGTTTTTCGTCAGTGTGGTGATTTACAAAAA ATTCCAAATTCCCTGCCCTCTGCCTGCTCTGGATCACAACAACGGAAATCTGACGTTCAACAACACACTT CCGATTCACATGATCTCGCTGCCTAATGACTCGGAGAGCTCGGGTGTGAACTTCATGATGGATTACGCTC ACCACAACCCAGCTGGGCTGGATGAGAAGCAGGTCGCAGGCCCTCTTCACAGCAATGGCGTGGAGTACGA AGCCCAGGGTGCTGAGAAATGCCAACCAAAATACTTTGTGTTCAATTCCCGGACGGCCTATGCAATCCCA ATCCTGGCTTTTGCTTTTGTCTGCCACCCTGAGGTCCTTCCCATCTACAGCGAGCTTAAAGATCGATCCC GCAGAAAGATGCAGACGGTGTCCAACATTTCCATCTCAGGCATGCTCGTCATGTACCTTCTTGCGGCCCT CTTTGGTTATCTGAGCTTCTACGGGGACGTTGAAGACGAGCTGCTGCATGCTTACAGCAAGGTCTACACA TTTGATACGGCTCTTCTCATGGTGCGCCTGGCAGTCCTGGTGGCAGTGACACTGACCGTGCCCATCGTGC TGTTCCCGATCCGTACTTCGGTGATCACACTGCTGTTTCCAAGGAAACCCTTCAGCTGGCTGAAGCATTT CGGGATCGCTGCAATCATCATCGCACTCAACAACATCCTGGTCATCCTCGTGCCTACCATCAAATACATC TTTGGATTCATAGGGGCTTCTTCTGCCACTATGCTGATTTTCATTCTTCCGGCTGCGTTTTATCTCAAGC TCGTCAAGAAAGAACCTCTAAGATCACCCCAGAAGATTGGGGCTTTGGTCTTCCTTGTGACTGGAATTAT TTTCATGATGGGAAGCATGGCGCTCATTATACTCGACTGGATCTACAACCCGCCGAATCCCAATCACCAC TAATCCCGGGGAGACGCGTCTCCACTGGAAACAGCTGAAATTGTCTGAAGGACATTTTAGTTGTCTTGAT TGGGATGTTAGTCTGAGGAATTAGCAAGATTCCAAAGACGTTTTTCTAGCTCTATCATGGGATACTTGTG GAAGAGAAAATTATGGGTTTTGTTGGGAATGGTTTTGTTGGGAATGGTGAAGGATGCATTAAAAATTCTG TGGCACACATTTTAACCCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4195569 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4195569 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7147 same embryo
	EMAGE:7150 same embryo
	EMAGE:7146 same embryo
	EMAGE:7149 same embryo
	EMAGE:7145 same embryo
	EurExpress:euxassay_004567 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source