EMAGE mouse gene expression spatial database

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EMAGE:7149

Ifitm3 interferon induced transmembrane protein 3 ( MGI:1913391)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004558. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004558_01	euxassay_004558_02	euxassay_004558_03	euxassay_004558_04

euxassay_004558_05	euxassay_004558_06	euxassay_004558_07	euxassay_004558_08	euxassay_004558_09

euxassay_004558_10	euxassay_004558_11	euxassay_004558_12	euxassay_004558_13	euxassay_004558_14

euxassay_004558_15	euxassay_004558_16	euxassay_004558_17	euxassay_004558_18	euxassay_004558_19

euxassay_004558_20	euxassay_004558_21	euxassay_004558_22	euxassay_004558_23	euxassay_004558_24

euxassay_004558_25	euxassay_004558_26	euxassay_004558_27	euxassay_004558_28

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7149_wholemount_strong.wlz

7149_wholemount_moderate.wlz

7149_wholemount_weak.wlz

7149_wholemount_possible.wlz

7149_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7149_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

regional

strong expression: see section 12 13 14 15 16 17 18

submandibular gland primordium

moderate

regional

moderate expression: see section 07 08 09 10 11 18 19 20 21 22

pancreas

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5825
Entity Detected:	Ifitm3, interferon induced transmembrane protein 3 ( MGI:1913391)
Sequence:	sense strand is shown >T5825 CCCGCAGCGCGCTCCATCCTTTGCCCTTCAGTGCTGCCTTTGCTCCGCACCATGAACCACACTTCTCAAG CCTTCATCACCGCTGCCAGTGGAGGACAGCCCCCAAACTACGAAAGAATCAAGGAAGAATATGAGGTGGC TGAGATGGGGGCACCGCACGGATCGGCTTCTGTCAGAACTACTGTGATCAACATGCCCAGAGAGGTGTCG GTGCCTGACCATGTGGTCTGGTCCCTGTTCAATACACTCTTCATGAACTTCTGCTGCCTGGGCTTCATAG CCTATGCCTACTCCGTGAAGTCTAGGGATCGGAAGATGGTGGGTGATGTGACTGGAGCCCAGGCCTACGC CTCCACTGCTAAGTGCCTGAACATCAGCACCTTGGTCCTCAGCATCCTGATGGTTGTTATCACCATTGTT AGTGTCATCATCATTGTTCTTAACGCTCAAAACCTTCACACTTAATAGAGGATTCCGACTTCCGGTCCTG AAGTGCTTCACCCTCCGCAGCTGCGTCCCTCCTTGCCCCTCCCTACACGCAGGTGTAACACTCATTTATC TATCCACAGTGGATTCAATAAAGTGCACTTGATAACCACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA A
Notes:	The probe template was PCR amplified from IMAGE:3152819 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3152819 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7147 same embryo
	EMAGE:7150 same embryo
	EMAGE:7146 same embryo
	EMAGE:7148 same embryo
	EMAGE:7145 same embryo
	EurExpress:euxassay_004558 same experiment
	MGI:4825520 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source